LOCUS       sequence-109065-        9612 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  generating transgenic TRIC flies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9612)
  AUTHORS   Gao XJ, Riabinina O, Li J, Potter CJ, Clandinin TR, Luo L
  TITLE     A transcriptional reporter of intracellular Ca in Drosophila.
  JOURNAL   Nat Neurosci. 2015 May 11. doi: 10.1038/nn.4016.
  PUBMED    25961791
REFERENCE   2  (bases 1 to 9612)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2015 May 11. doi: 10.1038/nn.4016."
COMMENT     Sequence Label: pQUAST-M13::GAL4DBDo
FEATURES             Location/Qualifiers
     source          1..9612
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(235..823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(315..334)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(994..1785,1786..1854))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1617..1636
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1855..1959)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2027..2045
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2083..2105)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2205..2224
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(2761..2791,2792..2993)
                     /label=P element 3' end
                     /note="P element 3' end"
     promoter        3189..3427
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     3374..3395
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             3495..3512
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             3516..3548
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(3552..3560,3561..3575)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      3582..3591
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3603..3659
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     CDS             3702..4142
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     intron          4241..4306
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4436..4456
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4728..4862
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4765..4784)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4819..4838
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4889..4907)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(5367..5438,5833..6106,6181..6835,6897..7212,7433..7564,
                     7635..8249)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(9027..9581,9582..9612)
                     /label=P element 5' end
ORIGIN
        1 ggccagaccc acgtagtcca gcggcagatc ggcggcggag aagttaagcg tctccaggat
       61 gaccttgccc gaactggggc acgtggtgtt cgacgatgtg cagctaattt cgcccggctc
      121 cacgtccgcc cattggttaa tcagcagacc ctcgttggcg taacggaacc atgagaggta
      181 cgacaaccat ttgaggtata ctggcaccga gcccgagttc aagaagaagg cgtttttcca
      241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      361 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      421 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      481 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      541 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      601 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      661 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      721 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      781 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      841 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
      901 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      961 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1021 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1081 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1141 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1201 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1261 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1321 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1381 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1441 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1501 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1561 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     1621 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1681 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1741 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1801 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1861 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1921 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1981 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     2041 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     2101 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     2161 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     2221 tgtactgaga gtgcaccata tgcggtgtga aataccgcac cgaatcgcgc ggaactaacg
     2281 acagtcgctc caaggtcgtc gaacaaaagg tgaatgtgtt gcggagagcg ggtgggagac
     2341 agcgaaagag caactacgaa acgtggtgtg gtggaggtga attatgaaga gggcgcgcga
     2401 tttgaaaagt atgtatataa aaaatatatc ccggtgtttt atgtagcgat aaacgagttt
     2461 ttgatgtaag gtatgcaggt gtgtaagtct tttggttaga agacaaatcc aaagtctact
     2521 tgtggggatg ttcgaagggg aaatacttgt attctatagg tcatatcttg tttttattgg
     2581 cacaaatata attacattag ctttttgagg gggcaataaa cagtaaacac gatggtaata
     2641 atggtaaaaa aaaaaacaag cagttatttc ggatatatgt cggctactcc ttgcgtcggg
     2701 cccgaagtct tagagccaga tatgcgagca cccggaagct cacgatgaga atggccagac
     2761 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
     2821 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
     2881 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
     2941 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttg
     3001 gctgcatcca acgcgttggg agctctccgg atccaagctt ggatccgggt aatcgcttat
     3061 cctcggataa acaattatcc tcacgggtaa tcgcttatcc gctcgggtaa tcgcttatcc
     3121 tcgggtaatc gcttatcctt aagcttgata tcgaattcct gcagcccgag cggagactct
     3181 agcgagcgcc ggagtataaa tagaggcgct tcgtctacgg agcgacaatt caattcaaac
     3241 aagcaaagtg aacacgtcgc taagcgaaag ctaagcaaat aaacaagcgc agctgaacaa
     3301 gctaaacaat ctgcagtaaa gtgcaagtta aagtgaatca attaaaagta accagcaacc
     3361 aagtaaatca actgcaacta ctgaaatctg ccaagaagta attattgaat acaagaagag
     3421 aactctgaat agggaattgg gaattcgtta acagatcggg ggatcaattc gttaacagat
     3481 ccaccatggg ttctcatcat catcatcatc atggtatggc tagcatgact ggtggacagc
     3541 aaatgggtcg ggatctgtac gacgatgacg ataaggatct cgccaccatg gtcgactcat
     3601 cacgtcgtaa gtggaataag acaggtcacg cagtcagagc tataggtcgg ctgagctcat
     3661 ctagcggagg aggtggtgga ggtggaggtg gaggtactag tatgaagctg ctgagtagta
     3721 ttgaacaagc gtgtgacata tgccgactta agaagctgaa gtgcagcaag gaaaagccga
     3781 agtgcgccaa atgtcttaag aacaattggg agtgccggta ctcgccaaag acgaagcgaa
     3841 gccctttgac acgcgctcat ttgactgaag tggagagtcg cctggagcgc cttgagcagt
     3901 tgttcctcct catttttccc cgtgaagacc tcgacatgat actgaagatg gatagtctcc
     3961 aagatattaa ggccctgctc actggcctgt ttgttcagga taacgtcaac aaggatgccg
     4021 tgacggaccg ccttgcctcg gtggagacag atatgccact taccttgcgg cagcatcgca
     4081 tctcggctac cagcagtagt gaagaaagtt ccaataaggg tcaacggcaa ctgacggtat
     4141 cgtaactcga gggtacctct agaggatctt tgtgaaggaa ccttacttct gtggtgtgac
     4201 ataattggac aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag
     4261 tgtataatgt gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg
     4321 aactgatgaa tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga
     4381 agaaatgcca tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa
     4441 aaagaagaga aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag
     4501 tcatgctgtg tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa
     4561 agctgcactg ctatacaaga aaattatgga aaaatatttg atgtatagtg ccttgactag
     4621 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     4681 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     4741 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     4801 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     4861 tcggatccac tagaaggcct tagtatgtat gtaagttaat aaaacccttt tttggagaat
     4921 gtagatttaa aaaaacatat ttttttttta ttttttactg cactggacat cattgaactt
     4981 atctgatcag ttttaaattt acttcgatcc aagggtattt gaagtaccag gttctttcga
     5041 ttacctctca ctcaaaatga cattccactc aaagtcagcg ctgtttgcct ccttctctgt
     5101 ccacagaaat atcgccgtct ctttcgccgc tgcgtccgct atctctttcg ccaccgtttg
     5161 tagcgttacc tagcgtcaat gtccgccttc agttgcactt tgtcagcggt ttcgtgacga
     5221 agctccaagc ggtttacgcc atcaattaaa cacaaagtgc tgtgccaaaa ctcctctcgc
     5281 ttcttatttt tgtttgtttt ttgagtgatt ggggtggtga ttggttttgg gtgggtaagc
     5341 aggggaaagt gtgaaaaatc ccggcaatgg gccaagagga tcaggagcta ttaattcgcg
     5401 gaggcagcaa acacccatct gccgagcatc tgaacaatgt gagtagtaca tgtgcataca
     5461 tcttaagttc acttgatcta taggaactgc gattgcaaca tcaaattgtc tgcggcgtga
     5521 gaactgcgac ccacaaaaat cccaaaccgc aatcgcacaa acaaatagtg acacgaaaca
     5581 gattattctg gtagctgtgc tcgctatata agacaatttt taagatcata tcatgatcaa
     5641 gacatctaaa ggcattcatt ttcgactaca ttctttttta caaaaaatat aacaaccaga
     5701 tattttaagc tgatcctaga tgcacaaaaa ataaataaaa gtataaacct acttcgtagg
     5761 atacttcgtt ttgttcgggg ttagatgagc ataacgcttg tagttgatat ttgagatccc
     5821 ctatcattgc agggtgacag cggagcggct tcgcagagct gcattaacca gggcttcggg
     5881 caggccaaaa actacggcac gctcctgcca cccagtccgc cggaggactc cggttcaggg
     5941 agcggccaac tagccgagaa cctcacctat gcctggcaca atatggacat ctttggggcg
     6001 gtcaatcagc cgggctccgg atggcggcag ctggtcaacc ggacacgcgg actattctgc
     6061 aacgagcgac acataccggc gcccaggaaa catttgctca agaacggtga gtttctattc
     6121 gcagtcggct gatctgtgtg aaatcttaat aaagggtcca attaccaatt tgaaactcag
     6181 tttgcggcgt ggcctatccg ggcgaacttt tggccgtgat gggcagttcc ggtgccggaa
     6241 agacgaccct gctgaatgcc cttgcctttc gatcgccgca gggcatccaa gtatcgccat
     6301 ccgggatgcg actgctcaat ggccaacctg tggacgccaa ggagatgcag gccaggtgcg
     6361 cctatgtcca gcaggatgac ctctttatcg gctccctaac ggccagggaa cacctgattt
     6421 tccaggccat ggtgcggatg ccacgacatc tgacctatcg gcagcgagtg gcccgcgtgg
     6481 atcaggtgat ccaggagctt tcgctcagca aatgtcagca cacgatcatc ggtgtgcccg
     6541 gcagggtgaa aggtctgtcc ggcggagaaa ggaagcgtct ggcattcgcc tccgaggcac
     6601 taaccgatcc gccgcttctg atctgcgatg agcccacctc cggactggac tcatttaccg
     6661 cccacagcgt cgtccaggtg ctgaagaagc tgtcgcagaa gggcaagacc gtcatcctga
     6721 ccattcatca gccgtcttcc gagctgtttg agctctttga caagatcctt ctgatggccg
     6781 agggcagggt agctttcttg ggcactccca gcgaagccgt cgacttcttt tcctagtgag
     6841 ttcgatgtgt ttattaaggg tatctagcat tacattacat ctcaactcct atccagcgtg
     6901 ggtgcccagt gtcctaccaa ctacaatccg gcggactttt acgtacaggt gttggccgtt
     6961 gtgcccggac gggagatcga gtcccgtgat cggatcgcca agatatgcga caattttgct
     7021 attagcaaag tagcccggga tatggagcag ttgttggcca ccaaaaattt ggagaagcca
     7081 ctggagcagc cggagaatgg gtacacctac aaggccacct ggttcatgca gttccgggcg
     7141 gtcctgtggc gatcctggct gtcggtgctc aaggaaccac tcctcgtaaa agtgcgactt
     7201 attcagacaa cggtgagtgg ttccagtgga aacaaatgat ataacgctta caattcttgg
     7261 aaacaaattc gctagatttt agttagaatt gcctgattcc acacccttct tagttttttt
     7321 caatgagatg tatagtttat agttttgcag aaaataaata aatttcattt aactcgcgaa
     7381 catgttgaag atatgaatat taatgagatg cgagtaacat tttaatttgc agatggttgc
     7441 catcttgatt ggcctcatct ttttgggcca acaactcacg caagtgggcg tgatgaatat
     7501 caacggagcc atcttcctct tcctgaccaa catgaccttt caaaacgtct ttgccacgat
     7561 aaatgtaagt cttgtttaga atacatttgc atattaataa tttactaact ttctaatgaa
     7621 tcgattcgat ttaggtgttc acctcagagc tgccagtttt tatgagggag gcccgaagtc
     7681 gactttatcg ctgtgacaca tactttctgg gcaaaacgat tgccgaatta ccgctttttc
     7741 tcacagtgcc actggtcttc acggcgattg cctatccgat gatcggactg cgggccggag
     7801 tgctgcactt cttcaactgc ctggcgctgg tcactctggt ggccaatgtg tcaacgtcct
     7861 tcggatatct aatatcctgc gccagctcct cgacctcgat ggcgctgtct gtgggtccgc
     7921 cggttatcat accattcctg ctctttggcg gcttcttctt gaactcgggc tcggtgccag
     7981 tatacctcaa atggttgtcg tacctctcat ggttccgtta cgccaacgag ggtctgctga
     8041 ttaaccaatg ggcggacgtg gagccgggcg aaattagctg cacatcgtcg aacaccacgt
     8101 gccccagttc gggcaaggtc atcctggaga cgcttaactt ctccgccgcc gatctgccgc
     8161 tggactacgt gggtctggcc attctcatcg tgagcttccg ggtgctcgca tatctggctc
     8221 taagacttcg ggcccgacgc aaggagtagc cgacatatat ccgaaataac tgcttgtttt
     8281 tttttttacc attattacca tcgtgtttac tgtttattgc cccctcaaaa agctaatgta
     8341 attatatttg tgccaataaa aacaagatat gacctataga atacaagtat ttccccttcg
     8401 aacatcccca caagtagact ttggatttgt cttctaacca aaagacttac acacctgcat
     8461 accttacatc aaaaactcgt ttatcgctac ataaaacacc gggatatatt ttttatatac
     8521 atacttttca aatcgcgcgc cctcttcata attcacctcc accacaccac gtttcgtagt
     8581 tgctctttcg ctgtctccca cccgctctcc gcaacacatt caccttttgt tcgacgacct
     8641 tggagcgact gtcgttagtt ccgcgcgatt cggttcgctc aaatggttcc gagtggttca
     8701 tttcgtctca atagaaatta gtaataaata tttgtatgta caatttattt gctccaatat
     8761 atttgtatat atttccctca cagctatatt tattctaatt taatattatg actttttaag
     8821 gtaatttttt gtgacctgtt cggagtgatt agcgttacaa tttgaactga aagtgacatc
     8881 cagtgtttgt tccttgtgta gatgcatctc aaaaaaatgg tgggcataat agtgttgttt
     8941 atatatatca aaaataacaa ctataataat aagaatacat ttaatttaga aaatgcttgg
     9001 atttcactgg aactagaatt aattcggctg ctgctctaaa cgacgcattt cgtactccaa
     9061 agtacgaatt ttttccctca agctcttatt ttcattaaac aatgaacagg acctaacgca
     9121 cagtcacgtt attgtttaca taaatgattt tttttactat tcaaacttac tctgtttgtg
     9181 tactcccact ggtatagcct tcttttatct tttctggttc aggctctatc actttactag
     9241 gtacggcatc tgcgttgagt cgcctccttt taaatgtctg accttttgca ggtgcagcct
     9301 tccactgcga atcattaaag tgggtatcac aaatttggga gttttcacca aggctgcacc
     9361 caaggctctg ctcccacaat tttctcttaa tagcacactt cggcacgtga attaatttta
     9421 ctccagtcac agctttgcag caaaatttgc aatatttcat ttttttttat tccacgtaag
     9481 ggttaatgtt ttcaaaaaaa aattcgtccg cacacaacct ttcctctcaa caagcaaacg
     9541 tgcactgaat ttaagtgtat acttcggtaa gcttcggcta tcgacgggac caccttatgt
     9601 tatttcatca tg
//
