LOCUS       sequence-109082-         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian gRNA expression vector also expressing CIB1-dCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Chen Z, Li W, Qiu F, Huang Q, Jiang Z, Ye J, Cheng P, Low C, Guo Y, 
            Yi X, Chen W, Yu Y, Han Y, Wu J, Jin S, Kong D, Huang J
  TITLE     Aspirin cooperates with p300 to activate the acetylation of H3K9 and
            promote FasL-mediated apoptosis of cancer stem-like cells in 
            colorectal cancer.
  JOURNAL   Theranostics. 2018 Aug 7;8(16):4447-4461. doi: 10.7150/thno.24284. 
            eCollection 2018.
  PUBMED    30214631
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.7150/thno.24284"; journalName: "Theranostics"; date: "2018-08-7-
            7"; volume: "8"; issue: "16"; pages: "4447-4461"
COMMENT     Sequence Label: px330-CIB1-dCas9
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..51
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        32..50
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      58..67
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             67..132
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             139..159
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 accgtcagat ccgctagaga tccgcggccg ctaatacgac tcactatagg gagagccgcc
       61 accatggact acaaagacca tgacggtgat tataaagatc atgacatcga ttacaaggat
      121 gacgatgaca agatggcccc caagaagaag aggaaggtgg gcattcaccg cggggtacct
      181 ggaggttctg gatccaacgg cgcgattggt ggggatttgc tgcttaactt tcccgacatg
      241 tccgtgttgg aacgtcagcg cgcacatttg aagtatctta accccacctt cgactccccg
      301 ttggccgggt tctttgcgga ctcatctatg attacgggag gggaaatgga cagctacctc
      361 tcaacggccg gattgaatct tccgatgatg tatggagaaa ccactgtaga aggcgactcg
      421 cgactctcga tttcgcctga aacgacgctg ggaacaggga acttcaaggc ggcgaaattc
      481 gacacggaga caaaagattg caacgaagcg gcgaagaaaa tgaccatgaa tcgcgatgat
      541 ctggtagagg agggagagga ggaaaagtcg aagattactg aacagaacaa tgggtctacc
      601 aaaagtatca aaaagatgaa gcacaaagct aagaaagaag agaacaattt cagcaatgac
      661 agcagtaaag tcacaaaaga actggagaaa acggattaca ttcacgtgag ggcgcgacga
      721 gggcaggcta cagattcaca ttcaattgcg gagagagtac ggagagagaa aatctcagaa
      781 aggatgaagt tcctccaaga ccttgtgcca ggttgtgaca agatcacagg caaagcagga
      841 atgctggatg agatcatcaa ctacgtccaa tcgttgcaaa gacaaattga gtttctctcg
      901 atgaa
//
