LOCUS       sequence-109087-         973 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas based plant genome editing and gene regulation; expresses
            3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence
            (OsU3 promoter), Hyg resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ
  TITLE     A CRISPR/Cas9 toolkit for multiplex genome editing in plants.
  JOURNAL   BMC Plant Biol. 2014 Nov 29;14(1):327.
  PUBMED    25432517
REFERENCE   2  (bases 1 to 973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2014-11-29"; volume: "14(1)"; pages: "327"
COMMENT     Sequence Label: pHUE411
FEATURES             Location/Qualifiers
     source          1..973
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(307..327)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(334..399)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 atcgtcaacc ttcgccatct cgttggagaa aatctcctgc aggtagcaga tgcgattctt
       61 cctgcgcgtg taccgcctgc gggcggtgcg cttgagccgc gtagcctcag ccgtctcgcc
      121 gctgtcgaac aggagagcgc caatgagatt cttcttgatg gaatgccgat cggtgttgcc
      181 caggaccttg aacttctttg agggcacctt gtactcgtcg gtgatcacgg cccagccaac
      241 agagttagtc ccaatatcga ggccgatcga gtacttcttg tcagcagctg gcaccccgtg
      301 gatgccaacc ttcctcttct tcttcggagc catcttgtca tcatcatcct tgtaatcaat
      361 gtcgtggtcc ttgtaatccc cgtcgtggtc cttgtaatcc atctagtagg cctagggctg
      421 cagaagtaac accaaacaac agggtgagca tcgacaaaag aaacagtacc aagcaaataa
      481 atagcgtatg aaggcagggc taaaaaaatc cacatatagc tgctgcatat gccatcatcc
      541 aagtatatca agatcaaaat aattataaaa catacttgtt tattataata gataggtact
      601 caaggttaga gcatatgaat agatgctgca tatgccatca tgtatatgca tcagtaaaac
      661 ccacatcaac atgtatacct atcctagatc gatatttcca tccatcttaa actcgtaact
      721 atgaagatgt atgacacaca catacagttc caaaattaat aaatacacca ggtagtttga
      781 aacagtattc tactccgatc tagaacgaat gaacgaccgc ccaaccacac cacatcatca
      841 caaccaagcg aacaaaaagc atctctgtat atgcatcagt aaaacccgca tcaacatgta
      901 tacctatcct agatcgatat ttccatccat catcttcaat tcgtaactat gaatatgtat
      961 ggcacacaca tac
//
