LOCUS       sequence-109088-         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AND gate reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Yang L, Nielsen AA, Fernandez-Rodriguez J, McClune CJ, Laub MT, Lu 
            TK, Voigt CA
  TITLE     Permanent genetic memory with >1-byte capacity.
  JOURNAL   Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 
            2014 Oct 26.
  PUBMED    25344638
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3147"; journalName: "Nat Methods"; date: "2014-12"; 
            volume: "11"; issue: "12"; pages: "1261-6"
COMMENT     Sequence Label: pAND_Reporter
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(158..185)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(201..272)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     misc_RNA        354..403
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     RBS             434..445
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     complement(482..510)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ctggatctat caacaggagt ccaagcgagc tcgtaaactt ggtctgacag tttttacggt
       61 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
      121 tggataaccg tattaccgcc tttgagtgag ctataaacgc agaaaggccc acccgaaggt
      181 gagccagtgt gactctagta gagagcgttc accgacaaac aacagataaa acgaaaggcc
      241 cagtctttcg actgagcctt tcgttttatt tgatgcctgg ctctagtagc tcatgtatgt
      301 gtctacgcga gattctcgcc cgagaacttc tgcaaggcac tgctcttggc tagctgtcac
      361 cggatgtgct ttccggtctg atgagtccgt gaggacgaaa cagcctctac aaataatttt
      421 gtttaatcta gagaaagagg agaaatacta gatgagtaaa ggagaagaac ttttcactgg
      481 agttgtccca attcttgttg aattagatgg taatgttaat gggcacaaat tttctgtcag
      541 tggagagggt gaaggtgatg cagcatacgg aaaacttacc cttaaattta tttgcactac
      601 tggaaaacta cctgttccat ggccaacact tgtcactact ttgacttatg gtgttcaatg
      661 cttttcaaga tacccagatc atatgaaacg gcatgacttt ttcaagagtg ccatgcccga
      721 aggttatgta caggaaagaa ctatattttt caaagatgac gggaactata agacacgtgc
      781 tgaagtcaag tttgaaggtg atacacttgt taatagaatc gagttaaaag gtattgattt
      841 taaagaagat ggaaacattc ttggacacaa gttggaatac aactataact cacacaatgt
      901 atacatcatg gcagaca
//
