LOCUS       sequence-109090-         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCACS backbone (Amp), TRP1, contains an RFP flanked by BsaI sites 
            with CAGT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Agmon N, Mitchell LA, Cai Y, Ikushima S, Chuang J, Zheng A, Choi WJ,
            Martin JA, Caravelli K, Stracquadanio G, Boeke JD
  TITLE     Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae 
            Transcription Units.
  JOURNAL   ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub
            2015 Mar 23.
  PUBMED    25756291
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500372z";
            journalName: "ACS Synth Biol"; date: "2015-07-17- 17"; volume: "4"; 
            issue: "7"; pages: "853-9"
COMMENT     Sequence Label: pAV10.K4
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             64..75
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    93..109
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(117..123,124..141,142..147))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    162..183
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(832..848)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(833..849)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 ttccatacga actttgaaac gcatgaactc tttgataacg tcttcggagg aagccatcta
       61 gtatttctcc tctttctcta gtatgtgtga aattgttatc cgctcacaat tccacacaac
      121 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
      181 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
      241 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggtctca ttttttcaaa
      301 ccattctgtt ataagagata tgtaccatca atcgccgaca gcgataccct ctttaacctc
      361 tagtaagaag gacccggcac ttattgtaat acgtttaggg gaagtaggta aatctggacc
      421 ggtaatacgg cataacgatc catttcggac atcggggaca tttctttttt ttcctttatt
      481 attttttttt tctaatgtcc ttacgaattt caggctctaa ccttatttct tattaggacg
      541 tgtactcatt gccatcgcaa caataaaaat aaaacattaa taaaaaatta attagagcct
      601 gtaacataga gatcaacgct actgtagcgt tagaaggaca gtgagagagt ggcatgacaa
      661 ttaaagtttc ttccgattac ttcatgtcat ctgcattaca aaggaaggaa agatgtttcg
      721 aattatttcc cctttaaggt atttttgatg tgaaatttga ccccatactg tgaaaatcaa
      781 ctgctaagaa ctctttaatt aagggctgca ggaattcgat atcaagctta tcgataccgt
      841 cgacctcgag g
//
