LOCUS       sequence-109091-         807 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pUC19 backbone (Amp), contains an RFP flanked by BsaI sites with 
            CAGT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Agmon N, Mitchell LA, Cai Y, Ikushima S, Chuang J, Zheng A, Choi WJ,
            Martin JA, Caravelli K, Stracquadanio G, Boeke JD
  TITLE     Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae 
            Transcription Units.
  JOURNAL   ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub
            2015 Mar 23.
  PUBMED    25756291
REFERENCE   2  (bases 1 to 807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500372z";
            journalName: "ACS Synth Biol"; date: "2015-07-17- 17"; volume: "4"; 
            issue: "7"; pages: "853-9"
COMMENT     Sequence Label: pAV10
FEATURES             Location/Qualifiers
     source          1..807
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             65..76
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    94..110
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(118..124,125..142,143..148))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    163..184
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(350..369)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(351..369)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(388..404)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(388..404)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    412..428
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(436..442,443..460,461..466))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    481..502
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(619..636)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cttccatacg aactttgaaa cgcatgaact ctttgataac gtcttcggag gaagccatct
       61 agtatttctc ctctttctct agtatgtgtg aaattgttat ccgctcacaa ttccacacaa
      121 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
      181 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
      241 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggtctc tactgtgcgg
      301 ccgcgggccg gccgatcctc tagagtcgac ctgcaggcat gcaagctttc cctatagtga
      361 gtcgtattag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
      421 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
      481 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
      541 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
      601 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
      661 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
      721 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
      781 gctggcgttt ttccataggc tccgccc
//
