LOCUS       sequence-109095-        1029 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV-mediated expression of ChrimsonR-tdTomato under the Syn 
            promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA 
            signal. TdTomato is a codon diversified version..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Klapoetke NC, Murata Y, Kim SS, Pulver SR, Birdsey-Benson A, Cho YK,
            Morimoto TK, Chuong AS, Carpenter EJ, Tian Z, Wang J, Xie Y, Yan Z, 
            Zhang Y, Chow BY, Surek B, Melkonian M, Jayaraman V, 
            Constantine-Paton M, Wong GK, Boyden ES
  TITLE     Independent optical excitation of distinct neural populations.
  JOURNAL   Nat Methods. 2014 Mar;11(3):338-46. doi: 10.1038/nmeth.2836. Epub 
            2014 Feb 9.
  PUBMED    24509633
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2836"; journalName: "Nat Methods"; date: "2014-03"; 
            volume: "11"; issue: "3"; pages: "338-46"
COMMENT     Sequence Label: pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato]
FEATURES             Location/Qualifiers
     source          1..1029
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    37..70
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    133..166
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gtgtcgtgcc tgagagcgca gtcgagaaga attcccataa cttcgtataa agtatcctat
       61 acgaagttat atcaaaatag gaagaccaat gcttcaccat cgacccgaat tgccaagcat
      121 caccatcgac ccataacttc gtataatgta tgctatacga agttatacta gctagctcat
      181 tacttataca gctcatccat gccgtacaga aacagatggt ggcgtccttc agacctctca
      241 tactgttcca caatggtgta gtcttcattg tgggatgtga tatccagctt ggtgtccaca
      301 tagtaatagc cgggcagctg cactggtttc ttggccatgt agatggtttt gaactccacc
      361 aggtagtggc cgccgtcttt cagcttcagg gcctggtgga tttcgccctt cagcactccg
      421 tctctaggat acagccgttc agtgctggcc tcccacccca ttgttttctt ctgcatcacg
      481 ggcccatcgg gtggaaaatt ggtgccgcgc attttgactt tgtagatcag agtgccatcc
      541 tgcagagagg agtcctgtgt cacggtcacc aggcctccat cttcaaaatt catcactctc
      601 tcccacttaa agccctctgg gaaagacagt ttcttgtaat caggaatgtc agcgggatgc
      661 ttgacatagg ccttacttcc gtacataaac tgaggagaca ggatatccca agcgaaaggc
      721 agtggtcctc ccttggtcac tttcagcttg gcagtctgtg tgccctcgta aggcctccct
      781 tcgccctctc cttcaatctc aaattcgtgc ccattcatgc tgccctccat gcgcacctta
      841 aacctcatga actctttgat cacagccata ttgttatcct cgcttgaggc ggttccacta
      901 gaccctgagc cggtacttcc agtcccatgg cccaggaaca gatggtgtct cccctcgctc
      961 cgttcatact gctccacaat agtgtagtcc tcgttgtgtg aagtgatatc cagttttgtg
     1021 tccacgtaa
//
