LOCUS       sequence-109109-         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV-mediated expression of CoChR-GFP under the Syn promoter, in 
            floxed/reversed (Cre-dependent) manner. Using bGHpA signal..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Klapoetke NC, Murata Y, Kim SS, Pulver SR, Birdsey-Benson A, Cho YK,
            Morimoto TK, Chuong AS, Carpenter EJ, Tian Z, Wang J, Xie Y, Yan Z, 
            Zhang Y, Chow BY, Surek B, Melkonian M, Jayaraman V, 
            Constantine-Paton M, Wong GK, Boyden ES
  TITLE     Independent optical excitation of distinct neural populations.
  JOURNAL   Nat Methods. 2014 Mar;11(3):338-46. doi: 10.1038/nmeth.2836. Epub 
            2014 Feb 9.
  PUBMED    24509633
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2836"; journalName: "Nat Methods"; date: "2014-03"; 
            volume: "11"; issue: "3"; pages: "338-46"
COMMENT     Sequence Label: pAAV-Syn-FLEX-rc[CoChR-GFP]
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    71..104
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    175..208
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 gtcaatcttt cacaaatttt gtaatccaga ggttgattat cgataagctt cgcgaacctc
       61 ttcgagggac ataacttcgt ataatgtatg ctatacgaag ttatgggtcg atggtgagat
      121 ctggactaga gggtcgatgg tgatgcttgg atcaaaatag gaagaatccc aatgataact
      181 tcgtataaag tatcctatac gaagttatac taggtaccta ggccgcgccg ccaccatgct
      241 gggaaacggc agcgccattg tgcctatcga ccagtgcttt tgcctggctt ggaccgacag
      301 cctgggaagc gatacagagc agctggtggc caacatcctc cagtggttcg ccttcggctt
      361 cagcatcctg atcctgatgt tctacgccta ccagacttgg agagccactt gcggttggga
      421 ggaggtctac gtctgttgcg tcgagctgac caaggtcatc atcgagttct tccacgagtt
      481 cgacgacccc agcatgctgt acctggctaa cggacaccga gtccagtggc tgagatacgc
      541 agagtggctg ctgacttgtc ccgtcatcct gatccacctg agcaacctga ccggcctgaa
      601 ggacgactac agcaagcgga ccatgaggct gctggtgtca gacgtgggaa ccatcgtgtg
      661 gggagctaca agcgccatga gcacaggcta cgtcaaggtc atcttcttcg tgctgggttg
      721 catctacggc gccaacacct tcttccacgc cgccaaggtg tatatcgaga gctaccacgt
      781 ggtgccaaag ggcagaccta gaaccgtcgt gcggatcatg gcttggctgt tcttcctgtc
      841 ttggggcatg ttccccgtgc tgttcgtcgt gggaccagaa ggattcgacg cc
//
