LOCUS       sequence-109123-         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondria, Excitation: 513, Emission: 527.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  TITLE     Davidson Yellow fluorescent proteins
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: YPet-Mito-7
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             30..104
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      132..141
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(444..463)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tggtttagtg aaccgtcaga tccgctagca tgtccgtcct gacgccgctg ctgctgcggg
       61 gcttgacagg ctcggcccgg cggctcccag tgccgcgcgc caagatccat tcgttggggg
      121 atccaccggt cgccaccatg gtgagcaaag gcgaagagct gttcaccggc gtggtgccca
      181 tcctggtgga gctggacggc gacgtgaacg gccacaagtt cagcgtgagc ggcgagggcg
      241 agggcgacgc cacctacggc aagctgaccc tgaagctgct gtgcaccacc ggcaagctgc
      301 ccgtgccctg gcccaccctg gtgaccaccc tgggctacgg cgtgcagtgc ttcgcccggt
      361 accccgacca catgaagcag cacgacttct tcaagagcgc catgcccgag ggctacgtgc
      421 aggagcggac catcttcttc aaggacgacg gcaactacaa gacccgggcc gaggtgaagt
      481 tcgagggcga caccctggtg aaccggatcg agctgaaggg catcgacttc aaggaggacg
      541 gcaacatcct gggccacaag ctggagtaca actacaacag ccacaacgtg tacatcaccg
      601 ccgacaagca gaagaacggc atcaaggcca acttcaagat ccggcacaac atcgaggacg
      661 gcggcgtgca gctggccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
      721 tgctgcccga caaccactac ctgagctacc agagcgccct gttcaaggac cccaacgaga
      781 agcg
//
