LOCUS       sequence-109132-        1001 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses rtTA2sM2 and tTSkid in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Ochiai H, Sugawara T, Yamamoto T
  TITLE     Simultaneous live imaging of the transcription and nuclear position 
            of specific genes.
  JOURNAL   Nucleic Acids Res. 2015 Jun 19. pii: gkv624.
  PUBMED    26092696
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Jun 19. pii: gkv624."
COMMENT     Sequence Label: pBSK-Delta-B- CAG-rtTA2sM2-IRES-tTSkid-IRES-Neo
FEATURES             Location/Qualifiers
     source          1..1001
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     40..59
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     130..149
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        130..148
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(194..811,812..817,818..853,854..856,857..892,893..895,
                     896..931,932..940)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
ORIGIN
        1 accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc
       61 tgtctcatca ttttggcaaa gagctctctg gctaactaga gaacccactg cttactggct
      121 tatcgaaatt aatacgactc actataggga gacccaagct tggtatcgac ctcggatcgt
      181 cgctagcgat atcatgtcta gactggacaa gagcaaagtc ataaacggcg ctctggaatt
      241 actcaatgga gtcggtatcg aaggcctgac gacaaggaaa ctcgctcaaa agctgggagt
      301 tgagcagcct accctgtact ggcacgtgaa gaacaagcgg gccctgctcg atgccctgcc
      361 aatcgagatg ctggacaggc atcataccca cttctgcccc ctggaaggcg agtcatggca
      421 agactttctg cggaacaacg ccaagtcatt ccgctgtgct ctcctctcac atcgcgacgg
      481 ggctaaagtg catctcggca cccgcccaac agagaaacag tacgaaaccc tggaaaatca
      541 gctcgcgttc ctgtgtcagc aaggcttctc cctggagaac gcactgtacg ctctgtccgc
      601 cgtgggccac tttacactgg gctgcgtatt ggaggaacag gagcatcaag tagcaaaaga
      661 ggaaagagag acacctacca ccgattctat gcccccactt ctgagacaag caattgagct
      721 gttcgaccgg cagggagccg aacctgcctt ccttttcggc ctggaactaa tcatatgtgg
      781 cctggagaaa cagctaaagt gcgaaagcgg cgggccggcc gacgcccttg acgattttga
      841 cttagacatg ctcccagccg atgcccttga cgactttgac cttgatatgc tgcctgctga
      901 cgctcttgac gattttgacc ttgacatgct ccccgggtaa ctaagtaagg atccagtact
      961 acgcgtcgag catgcatcta gggcggccaa ttccgcccct c
//
