LOCUS       sequence-109133-         993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses rtTA2sM2 and tTSkid in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Ochiai H, Sugawara T, Yamamoto T
  TITLE     Simultaneous live imaging of the transcription and nuclear position 
            of specific genes.
  JOURNAL   Nucleic Acids Res. 2015 Jun 19. pii: gkv624.
  PUBMED    26092696
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Jun 19. pii: gkv624."
COMMENT     Sequence Label: pBSK-Delta-B- CAG-rtTA2sM2-IRES-tTSkid-IRES-Neo
FEATURES             Location/Qualifiers
     source          1..993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(152..955)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(263..282)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     873..892
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
       61 gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga
      121 ttatgatcag ttatctagag aattgatccc ctcagaagaa ctcgtcaaga aggcgataga
      181 aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag cggtcagccc
      241 attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc tgatagcggt
      301 ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt tccaccatga
      361 tattcggcaa gcaggcatcg ccatgggtca cgacgagatc atcgccgtcg ggcatgcgcg
      421 ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg tccagatcat
      481 cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt
      541 ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca
      601 tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc cccggcactt
      661 cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag
      721 gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcctgcagt tcattcaggg
      781 caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca
      841 cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat agcctctcca
      901 cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatggccgat cccatattgg
      961 ctgcaggaat tatcccgggt tgtggcaagc tta
//
