LOCUS       sequence-109134-         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses rtTA2sM2 and tTSkid in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Ochiai H, Sugawara T, Yamamoto T
  TITLE     Simultaneous live imaging of the transcription and nuclear position 
            of specific genes.
  JOURNAL   Nucleic Acids Res. 2015 Jun 19. pii: gkv624.
  PUBMED    26092696
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Jun 19. pii: gkv624."
COMMENT     Sequence Label: pBSK-Delta-B- CAG-rtTA2sM2-IRES-tTSkid-IRES-Neo
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(62..256,257..277,278..910))
                     /codon_start=1
                     /product="fusion of a hybrid tetracycline repressor TetR 
                     with a nuclear localization signal and a KRAB silencing 
                     domain"
                     /label=tTS
                     /note="silences Tet-responsive promoters in the absence but
                     not the presence of doxycycline"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     RNKQTLMNMLSEAILAKHHTRSAPLPTESWQQFLQENALSFRKALLVHRDGARLHIGTS
                     PTPPQFEQAEAQLRCLCDAGFSVEEALFILQSISHFTLGAVLEEQATNQIENNHVIDAA
                     PPLLQEAFNIQARTSAEMAFHFGLKSLIFGFSAQLDEKKHTPIEDGNKPKKKRKLAVSV
                     TFEDVAVLFTRDEWKKLDLSQRSLYREVMLENYSNLASMAGFLFTKPKVISLLQQGEDP
                     W"
     primer_bind     818..837
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 gcgaattaat tccagcacac tggatcagtt atctatgcgg ccgctcatta attaacctcg
       61 attaccaggg atcctctcct tgctgcaaca gggagatcac ctttggtttg gtaaacagga
      121 atcctgccat ggaggccagg ttgctgtaat tctccagcat cacctcacgg tacaggcttc
      181 tctgagacag atccagcttc ttccactcgt cccgagtaaa gagcacagcc acatcttcaa
      241 atgtcactga cactgctagc tttctcttct tttttggttt attaccatcc tcaatgggtg
      301 tatgcttttt ttcatctaac tgtgcagaaa atccaaatat taatgatttc agcccgaaat
      361 ggaaggccat ttcagcagag gttctcgcct gaatattaaa tgcctcttgt aataatggtg
      421 gtgcagcgtc tatcacatga ttattttcta tctggtttgt tgcttgctcc tctaatactg
      481 cacccaacgt aaaatggctg atagattgca gaatgaaaag agcctcctcg accgaaaacc
      541 ctgcatcgca tagacagcgt agttgcgcct ctgcttgttc aaactggggg ggcgtaggag
      601 aggtccctat atgcaatcgg gctccatcac gatggaccag taatgcttta cggaaactca
      661 gagcattttc ctggagaaac tgctgccaac tctcagtcgg taacggtgct gaacgggtgt
      721 gatgcttcgc cagtattgcc tctgaaagca tgttcataag agtctgcttg ttgcgcacgt
      781 gccaatacaa tgtaggctgc tctacaccta gcttctgggc gagtttacgg gttgttaaac
      841 cttcgattcc gacctcatta agcagctcta atgcgctgtt aatcacttta cttttatcta
      901 atctagacat ggtgaattcg gggccgcgga ggctggatcg gtcccggtgt cttctatgga
//
