LOCUS       sequence-109135-         760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses rtTA2sM2 and tTSkid in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Ochiai H, Sugawara T, Yamamoto T
  TITLE     Simultaneous live imaging of the transcription and nuclear position 
            of specific genes.
  JOURNAL   Nucleic Acids Res. 2015 Jun 19. pii: gkv624.
  PUBMED    26092696
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Jun 19. pii: gkv624."
COMMENT     Sequence Label: pBSK-Delta-B- CAG-rtTA2sM2-IRES-tTSkid-IRES-Neo
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(139..163)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    198..660
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(358..377)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     587..604
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 ctcattaagc agctctaatg cgctgttaat cactttactt ttatctaatc tagacatggt
       61 gaattcgggg ccgcggaggc tggatcggtc ccggtgtctt ctatggaggt caaaacagcg
      121 tggatggcgt ctccaggcga tctgacggtt cactaaacga gctcagtact cctagaggat
      181 cccgggttgt ggcaagctta tcatcgtgtt tttcaaagga aaaccacgtc cccgtggttc
      241 ggggggccta gacgtttttt taacctcgac taaacacatg taaagcatgt gcaccgaggc
      301 cccagatcag atcccataca atggggtacc ttctgggcat ccttcagccc cttgttgaat
      361 acgcttgagg agagccattt gactctttcc acaactatcc aactcacaac gtggcactgg
      421 ggttgtgccg cctttgcagg tgtatcttat acacgtggct tttggccgca gaggcacctg
      481 tcgccaggtg gggggttccg ctgcctgcaa agggtcgcta cagacgttgt ttgtcttcaa
      541 gaagcttcca gaggaactgc ttccttcacg acattcaaca gaccttgcat tcctttggcg
      601 agaggggaaa gacccctagg aatgctcgtc aagaagacag ggccaggttt ccgggccctc
      661 acattgccaa aagacggcaa tatggtggaa aataacatat agacaaacgc acaccggcct
      721 tattccaagc ggcttcggcc agtaacgtta gggggggggg
//
