LOCUS       sequence-109136-        9235 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses rtTA2sM2 and tTSkid in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9235)
  AUTHORS   Ochiai H, Sugawara T, Yamamoto T
  TITLE     Simultaneous live imaging of the transcription and nuclear position 
            of specific genes.
  JOURNAL   Nucleic Acids Res. 2015 Jun 19. pii: gkv624.
  PUBMED    26092696
REFERENCE   2  (bases 1 to 9235)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Jun 19. pii: gkv624."
COMMENT     Sequence Label: pBSK-Delta-B- CAG-rtTA2sM2-IRES-tTSkid-IRES-Neo
FEATURES             Location/Qualifiers
     source          1..9235
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    754..875
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     819..838
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1005..1808)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1116..1135)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1726..1745
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     misc_feature    1844..2417
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2488..2507)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          2490..2719
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     CDS             complement(join(2805..2999,3000..3020,3021..3653))
                     /codon_start=1
                     /product="fusion of a hybrid tetracycline repressor TetR 
                     with a nuclear localization signal and a KRAB silencing 
                     domain"
                     /label=tTS
                     /note="silences Tet-responsive promoters in the absence but
                     not the presence of doxycycline"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     RNKQTLMNMLSEAILAKHHTRSAPLPTESWQQFLQENALSFRKALLVHRDGARLHIGTS
                     PTPPQFEQAEAQLRCLCDAGFSVEEALFILQSISHFTLGAVLEEQATNQIENNHVIDAA
                     PPLLQEAFNIQARTSAEMAFHFGLKSLIFGFSAQLDEKKHTPIEDGNKPKKKRKLAVSV
                     TFEDVAVLFTRDEWKKLDLSQRSLYREVMLENYSNLASMAGFLFTKPKVISLLQQGEDP
                     W"
     primer_bind     3561..3580
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     complement(3735..3759)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    3794..4367
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             complement(join(4438..4446,4447..4482,4483..4485,4486..4521,
                     4522..4524,4525..4560,4561..4566,4567..5184))
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(5230..5248)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5319..5338)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          5346..6354
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(5400..5422)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(6355..6630)
                     /label=chicken beta-actin promoter
     enhancer        6633..6936
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(7047..7067)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7047..7065)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7086..7102)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7086..7102)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7099..7121)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7110..7126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7134..7140,7141..7158,7159..7164))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7179..7200
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7317..7334)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7488..8076)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7568..7587)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8247..9038,9039..9107))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8870..8889
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9108..9212)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gccccccctc gaggtcgacg agacgggtat acagacatga taagatacat tgatgagttt
      721 ggacaaacca caactagaat gcactcgacg cgttaagata cattgatgag tttggacaaa
      781 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
      841 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
      901 tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat
      961 gtggtatggc tgattatgat cagttatcta gagaattgat cccctcagaa gaactcgtca
     1021 agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg
     1081 aagcggtcag cccattcgcc gccaagctct tcagcaatat cacgggtagc caacgctatg
     1141 tcctgatagc ggtccgccac acccagccgg ccacagtcga tgaatccaga aaagcggcca
     1201 ttttccacca tgatattcgg caagcaggca tcgccatggg tcacgacgag atcatcgccg
     1261 tcgggcatgc gcgccttgag cctggcgaac agttcggctg gcgcgagccc ctgatgctct
     1321 tcgtccagat catcctgatc gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg
     1381 cgatgtttcg cttggtggtc gaatgggcag gtagccggat caagcgtatg cagccgccgc
     1441 attgcatcag ccatgatgga tactttctcg gcaggagcaa ggtgagatga caggagatcc
     1501 tgccccggca cttcgcccaa tagcagccag tcccttcccg cttcagtgac aacgtcgagc
     1561 acagctgcgc aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc ctcgtcctgc
     1621 agttcattca gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct
     1681 gacagccgga acacggcggc atcagagcag ccgattgtct gttgtgccca gtcatagccg
     1741 aatagcctct ccacccaagc ggccggagaa cctgcgtgca atccatcttg ttcaatggcc
     1801 gatcccatat tggctgcagg aattatcccg ggttgtggca agcttatcat cgtgtttttc
     1861 aaaggaaaac cacgtccccg tggttcgggg ggcctagacg tttttttaac ctcgactaaa
     1921 cacatgtaaa gcatgtgcac cgaggcccca gatcagatcc catacaatgg ggtaccttct
     1981 gggcatcctt cagccccttg ttgaatacgc ttgaggagag ccatttgact ctttccacaa
     2041 ctatccaact cacaacgtgg cactggggtt gtgccgcctt tgcaggtgta tcttatacac
     2101 gtggcttttg gccgcagagg cacctgtcgc caggtggggg gttccgctgc ctgcaaaggg
     2161 tcgctacaga cgttgtttgt cttcaagaag cttccagagg aactgcttcc ttcacgacat
     2221 tcaacagacc ttgcattcct ttggcgagag gggaaagacc cctaggaatg ctcgtcaaga
     2281 agacagggcc aggtttccgg gccctcacat tgccaaaaga cggcaatatg gtggaaaata
     2341 acatatagac aaacgcacac cggccttatt ccaagcggct tcggccagta acgttagggg
     2401 ggggggaggg aaaagggggg gggggagagg ggcggaattg gccgccctag atgcatgctc
     2461 gacctgcagt tggacctggg agtggacacc tgtggagaga aaggcaaagt ggatgtcatt
     2521 gtcactcaag tgtatggcca gatctcaagc ctgccacacc tcaagcttga caacaaaaag
     2581 attgtctttt ctgaccagat ggacgcggcc accctcaaag gcatcaccgc gggccaggtg
     2641 aatatcaaat cctcctcgtt tttggaaact gacaatctta gcgcagaagt catgcccgct
     2701 tttgagaggg agtactcacc ccaacagctg gccctcgcag acagcgaatt aattccagca
     2761 cactggatca gttatctatg cggccgctca ttaattaacc tcgattacca gggatcctct
     2821 ccttgctgca acagggagat cacctttggt ttggtaaaca ggaatcctgc catggaggcc
     2881 aggttgctgt aattctccag catcacctca cggtacaggc ttctctgaga cagatccagc
     2941 ttcttccact cgtcccgagt aaagagcaca gccacatctt caaatgtcac tgacactgct
     3001 agctttctct tcttttttgg tttattacca tcctcaatgg gtgtatgctt tttttcatct
     3061 aactgtgcag aaaatccaaa tattaatgat ttcagcccga aatggaaggc catttcagca
     3121 gaggttctcg cctgaatatt aaatgcctct tgtaataatg gtggtgcagc gtctatcaca
     3181 tgattatttt ctatctggtt tgttgcttgc tcctctaata ctgcacccaa cgtaaaatgg
     3241 ctgatagatt gcagaatgaa aagagcctcc tcgaccgaaa accctgcatc gcatagacag
     3301 cgtagttgcg cctctgcttg ttcaaactgg gggggcgtag gagaggtccc tatatgcaat
     3361 cgggctccat cacgatggac cagtaatgct ttacggaaac tcagagcatt ttcctggaga
     3421 aactgctgcc aactctcagt cggtaacggt gctgaacggg tgtgatgctt cgccagtatt
     3481 gcctctgaaa gcatgttcat aagagtctgc ttgttgcgca cgtgccaata caatgtaggc
     3541 tgctctacac ctagcttctg ggcgagttta cgggttgtta aaccttcgat tccgacctca
     3601 ttaagcagct ctaatgcgct gttaatcact ttacttttat ctaatctaga catggtgaat
     3661 tcggggccgc ggaggctgga tcggtcccgg tgtcttctat ggaggtcaaa acagcgtgga
     3721 tggcgtctcc aggcgatctg acggttcact aaacgagctc agtactccta gaggatcccg
     3781 ggttgtggca agcttatcat cgtgtttttc aaaggaaaac cacgtccccg tggttcgggg
     3841 ggcctagacg tttttttaac ctcgactaaa cacatgtaaa gcatgtgcac cgaggcccca
     3901 gatcagatcc catacaatgg ggtaccttct gggcatcctt cagccccttg ttgaatacgc
     3961 ttgaggagag ccatttgact ctttccacaa ctatccaact cacaacgtgg cactggggtt
     4021 gtgccgcctt tgcaggtgta tcttatacac gtggcttttg gccgcagagg cacctgtcgc
     4081 caggtggggg gttccgctgc ctgcaaaggg tcgctacaga cgttgtttgt cttcaagaag
     4141 cttccagagg aactgcttcc ttcacgacat tcaacagacc ttgcattcct ttggcgagag
     4201 gggaaagacc cctaggaatg ctcgtcaaga agacagggcc aggtttccgg gccctcacat
     4261 tgccaaaaga cggcaatatg gtggaaaata acatatagac aaacgcacac cggccttatt
     4321 ccaagcggct tcggccagta acgttagggg ggggggaggg aaaagggggg gggggagagg
     4381 ggcggaattg gccgccctag atgcatgctc gacgcgtagt actggatcct tacttagtta
     4441 cccggggagc atgtcaaggt caaaatcgtc aagagcgtca gcaggcagca tatcaaggtc
     4501 aaagtcgtca agggcatcgg ctgggagcat gtctaagtca aaatcgtcaa gggcgtcggc
     4561 cggcccgccg ctttcgcact ttagctgttt ctccaggcca catatgatta gttccaggcc
     4621 gaaaaggaag gcaggttcgg ctccctgccg gtcgaacagc tcaattgctt gtctcagaag
     4681 tgggggcata gaatcggtgg taggtgtctc tctttcctct tttgctactt gatgctcctg
     4741 ttcctccaat acgcagccca gtgtaaagtg gcccacggcg gacagagcgt acagtgcgtt
     4801 ctccagggag aagccttgct gacacaggaa cgcgagctga ttttccaggg tttcgtactg
     4861 tttctctgtt gggcgggtgc cgagatgcac tttagccccg tcgcgatgtg agaggagagc
     4921 acagcggaat gacttggcgt tgttccgcag aaagtcttgc catgactcgc cttccagggg
     4981 gcagaagtgg gtatgatgcc tgtccagcat ctcgattggc agggcatcga gcagggcccg
     5041 cttgttcttc acgtgccagt acagggtagg ctgctcaact cccagctttt gagcgagttt
     5101 ccttgtcgtc aggccttcga taccgactcc attgagtaat tccagagcgc cgtttatgac
     5161 tttgctcttg tccagtctag acatgatatc gctagcgacg atccgaggtc gataccaagc
     5221 ttgggtctcc ctatagtgag tcgtattaat ttcgataagc cagtaagcag tgggttctct
     5281 agttagccag agagctcttt gccaaaatga tgagacagca caataaccag cacgttgccc
     5341 aggagctgta ggaaaaagaa gaaggcatga acatggttag cagaggctct agagccgccg
     5401 gtcacacgcc agaagccgaa ccccgccctg ccccgtcccc cccgaaggca gccgtccccc
     5461 tgcggcagcc ccgaggctgg agatggagaa ggggacggcg gcgcggcgac gcacgaaggc
     5521 cctccccgcc catttccttc ctgccggcgc cgcaccgctt cgcccgcgcc cgctagaggg
     5581 ggtgcggcgg cgcctcccag atttcggctc cgccagattt gggacaaagg aagtccctgc
     5641 gccctctcgc acgattacca taaaaggcaa tggctgcggc tcgccgcgcc tcgacagccg
     5701 ccggcgctcc ggggccgccg cgcccctccc ccgagccctc cccggcccga ggcggccccg
     5761 ccccgcccgg cacccccacc tgccgccacc ccccgcccgg cacggcgagc cccgcgccac
     5821 gccccgcacg gagccccgca cccgaagccg ggccgtgctc agcaactcgg ggaggggggt
     5881 gcaggggggg gttacagccc gaccgccgcg cccacacccc ctgctcaccc ccccacgcac
     5941 acaccccgca cgcagccttt gttcccctcg cagccccccc gcaccgcggg gcaccgcccc
     6001 cggccgcgct cccctcgcgc acacgcggag cgcacaaagc cccgcgccgc gcccgcagcg
     6061 ctcacagccg ccgggcagcg cgggccgcac gcggcgctcc ccacgcacac acacacgcac
     6121 gcaccccccg agccgctccc ccccgcacaa agggccctcc cggagccctt taaggctttc
     6181 acgcagccac agaaaagaaa cgagccgtca ttaaaccaag cgctaattac agcccggagg
     6241 agaagggccg tcccgcccgc tcacctgtgg gagtaacgcg gtcagtcaga gccggggcgg
     6301 gcggcgcgag gcggcgcgga gcggggcacg gggcgaaggc aacgcagcga ctcccgcccg
     6361 ccgcgcgctt cgctttttat agggccgccg ccgccgccgc ctcgccataa aaggaaactt
     6421 tcggagcgcg ccgctctgat tggctgccgc cgcacctctc cgcctcgccc cgccccgccc
     6481 ctcgccccgc cccgccccgc ctggcgcgcg cccccccccc ccccgccccc atcgctgcac
     6541 aaaataatta aaaaataaat aaatacaaaa ttgggggtgg ggaggggggg gagatgggga
     6601 gagtgaagca gaacgtgggg ctcacctcga cccatggtaa tagcgatgac taatacgtag
     6661 atgtactgcc aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc
     6721 catttaccgt cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact
     6781 gccaagtggg cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt
     6841 ggcgttacta tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt
     6901 cagccaggcg ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga
     6961 actaatgacc ccgtaattga ttactattaa tacgtctcac tagttctaga gcggccgcca
     7021 ccgcggtgga gctccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa
     7081 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     7141 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     7201 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     7261 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     7321 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     7381 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     7441 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     7501 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     7561 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     7621 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     7681 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     7741 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     7801 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     7861 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     7921 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     7981 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     8041 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     8101 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     8161 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     8221 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     8281 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     8341 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcggct gccacgctca
     8401 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     8461 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     8521 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     8581 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     8641 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     8701 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     8761 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     8821 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     8881 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     8941 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     9001 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     9061 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     9121 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     9181 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccac
//
