LOCUS       sequence-109137-         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  An empty optimized gRNA expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Ochiai H, Sugawara T, Yamamoto T
  TITLE     Simultaneous live imaging of the transcription and nuclear position 
            of specific genes.
  JOURNAL   Nucleic Acids Res. 2015 Jun 19. pii: gkv624.
  PUBMED    26092696
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Jun 19. pii: gkv624."
COMMENT     Sequence Label: pKLV-U6gRNA-EF(BbsI)-PGKpuro2ABFP
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        98..346
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     98..118
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     269..288
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(532..555)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
       61 gggacagcag agatccagtt tggttagtac cgggcccgag ggcctatttc ccatgattcc
      121 ttcatatttg catatacgat acaaggctgt tagagagata attggaatta atttgactgt
      181 aaacacaaag atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt
      241 tgcagtttta aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta
      301 tttcgatttc ttggctttat atatcttgtg gaaaggacga aacaccgggt cttcgagaag
      361 acctgtttaa gagctatgct ggaaacagca tagcaagttt aaataaggct agtccgttat
      421 caacttgaaa aagtggcacc gagtcggtgc ttttttgttt tagagctaga aatagcaagt
      481 tggatccaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct
      541 ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc
      601 acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct
      661 actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga
      721 caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg
      781 aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct
      841 cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg
      901 ggcgcccgaa ggtcctccgg aggc
//
