LOCUS       sequence-109146-         647 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Non-integrating (episomal) expression of human OCT3/4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Okita K, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, Hong 
            H, Nakagawa M, Tanabe K, Tezuka KI, Shibata T, Kunisada T, Takahashi
            M, Takahashi J, Saji H, Yamanaka S
  TITLE     A more efficient method to generate integration-free human iPS 
            cells.
  JOURNAL   Nat Methods. 2011 May;8(5):409-12
  PUBMED    21460823
REFERENCE   2  (bases 1 to 647)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-05"; volume: "8(5)"; pages: "409-1"
COMMENT     Sequence Label: pCXLE-hOCT3/4
FEATURES             Location/Qualifiers
     source          1..647
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    109..142
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
       61 tgtcgtgcca gcggatctca attccgatca tattcaataa cccttaatat aacttcgtat
      121 aatgtatgct atacgaagtt attaggtctg aagaggagtt tacgtccagc caagcttagg
      181 atcaattctc atgtttgaca gcttatcatc gataagctga tcctcacagg ccgcacccag
      241 cttttcttcc gttgccccag tagcatctct gtctggtgac cttgaagagg aagaggaggg
      301 gtcccgagaa tccccatccc taccgtccag caaaaagggg gacgaggaat ttgaggcctg
      361 gcttgaggct caggacgcaa atcttgagga tgttcagcgg gagttttccg ggctgcgagt
      421 aattggtgat gaggacgagg atggttcgga ggatggggaa ttttcagacc tggatctgtc
      481 tgacagcgac catgaagggg atgagggtgg gggggctgtt ggagggggca ggagtctgca
      541 ctccctgtat tcactgagcg tcgtctaata aagatgtcta ttgatctctt ttagtgtgaa
      601 tcatgtctga cgaggggcca ggtacaggac ctggaaatgg cctagga
//
