LOCUS       sequence-109218-         752 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For replacement of VSG221 with a blasticidin resistence gene in the 
            VSG221 expression site in T.brucei..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 752)
  TITLE     pBSVSG221KOblast
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBSVSG221KOblast
FEATURES             Location/Qualifiers
     source          1..752
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tcgagtccta agcctaagtc taatcctaat cctaagccta acccccctaa ctaaccccct
       61 aactaaccta actaacctac ctaactaccc ccccccccca ttcctaccta cctacaaaaa
      121 aaacaggaaa aaagaaaaaa attttagttg ccagaaaata tttctagaca atcagacagt
      181 caacttacaa aacacagaac acaaaatctc ggctggtttt ggtgttaaaa tatatcaaaa
      241 agttttcaag tagcaaggaa aattttaaaa ggagggggga aatttgaggg gggaaattaa
      301 aaaagcaaaa ctgcaagcca aagaggggtc ttgctaatga caaaagaatt gctgcttcct
      361 gtggtgtttg tgtttccagt tttcccatct tttgcagtct catctgctac cttttttgcc
      421 tcctccttat ccaatgtgca ctttttgttt tccgcatcgt tatgccattt gcatggggat
      481 ttgcactcat tttggtcctt cttgttgcag ctgccttctg ttcctgctgc ttgttgttgg
      541 ctttcagcgg gcttgtgctt ctgggttaaa acttcaaatt tccctgctgt ttccattcgg
      601 taatagaagc ttgataattc ttagccctcc cacacataac cagagggcag caattcacga
      661 atcccaactg ccgtcggctg tccatcactg tccttcacta tggctttgat cccaggatgc
      721 agatcgagaa gcacctgtcg gcaccgtccg ca
//
