LOCUS       sequence-109221-         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of Acyl Carrier Protein (ACP) from S. aureus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  TITLE     Johnson Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET15b-ACP (from S. aureus)
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             33..50
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             60..77
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(370..388)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      384..431
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(606..611,612..628,629..634))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(661..679)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac agcagcggcc
       61 tggtgccgcg cggcagccat atggagaact tcgacaaggt aaaagacatc atcgtcgacc
      121 gtctgggtgt tgacgctgat aaagttaccg aggacgcatc ttttaaagac gatctgggtg
      181 ctgacagcct ggatattgcg gaactggtta tggaactgga agatgaattc ggcactgaaa
      241 tcccggatga agaagccgag aaaattaaca cggtgggcga tgcggtgaaa ttcatcaatt
      301 ccctggaaaa ataactcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg
      361 ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga
      421 ggggtttttt gctgaaagga ggaactatat ccggatatcc cgcaagaggc ccggcagtac
      481 cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag
      541 cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact
      601 accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg
      661 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
      721 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
      781 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcat taatattgaa
      841 a
//
