LOCUS       sequence-109222-         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of Acyl Carrier Protein (ACP) from S. aureus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  TITLE     Johnson Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET15b-ACP (from S. aureus)
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(253..270)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(280..297)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             316..338
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    353..377
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(377..396)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(378..396)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(480..499)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        705..782
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(802..821)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 agccggatcc tcgagttatt tttccaggga attgatgaat ttcaccgcat cgcccaccgt
       61 gttaattttc tcggcttctt catccgggat ttcagtgccg aattcatctt ccagttccat
      121 aaccagttcc gcaatatcca ggctgtcagc acccagatcg tctttaaaag atgcgtcctc
      181 ggtaacttta tcagcgtcaa cacccagacg gtcgacgatg atgtctttta ccttgtcgaa
      241 gttctccata tggctgccgc gcggcaccag gccgctgctg tgatgatgat gatgatggct
      301 gctgcccatg gtatatctcc ttcttaaagt taaacaaaat tatttctaga ggggaattgt
      361 tatccgctca caattcccct atagtgagtc gtattaattt cgcgggatcg agatctcgat
      421 cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc
      481 ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc
      541 ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc
      601 cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg
      661 cttcctaatg caggagtcgc ataagggaga gcgtcgagat cccggacacc atcgaatggc
      721 gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga gagtcaattc agggtggtga
      781 atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg
//
