LOCUS       sequence-109223-         651 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of Acyl Carrier Protein Synthase (ACPs) from S.
            pneumoniae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 651)
  TITLE     Johnson Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 651)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET15b-ACPs (from S. pneumonia)
FEATURES             Location/Qualifiers
     source          1..651
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(379..396)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(406..423)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             442..464
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    479..503
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(503..522)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(504..522)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(606..625)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gcagccggat ccctagcttt catgattttc ctccaaaatg acactggctg tcacaaactg
       61 atcggtgtgg ctgatagaca gccaaatctt tcctgaaaat ggtgcctgac taaaataagg
      121 cgccccacgt tcattgttca agacttccaa atcctgaaaa ccgagcttgc taatgcccgt
      181 tcccatagcc ttggaaaagg cctccttagc cgaccagcga ccagctaaat attctatttg
      241 cctgcgtcct ttgagactgg tgaagcgctc catttcctga gcggtcagta cacgcttagc
      301 aaatccttca tgtcgtgtaa ctgcgctttc tatcgaagcc aattcttcga tgtcaattcc
      361 gtgtccaact atcatatggc tgccgcgcgg caccaggccg ctgctgtgat gatgatgatg
      421 atggctgctg cccatggtat atctccttct taaagttaaa caaaattatt tctagagggg
      481 aattgttatc cgctcacaat tcccctatag tgagtcgtat taatttcgcg ggatcgagat
      541 ctcgatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc
      601 tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact t
//
