LOCUS       sequence-109224-         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of Acyl Carrier Protein Synthase (ACPs) from S.
            pneumoniae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  TITLE     Johnson Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET15b-ACPs (from S. pneumonia)
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             33..50
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             60..77
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(494..512)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      508..555
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(730..735,736..752,753..758))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(785..803)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac agcagcggcc
       61 tggtgccgcg cggcagccat atgatagttg gacacggaat tgacatcgaa gaattggctt
      121 cgatagaaag cgcagttaca cgacatgaag gatttgctaa gcgtgtactg accgctcagg
      181 aaatggagcg cttcaccagt ctcaaaggac gcaggcaaat agaatattta gctggtcgct
      241 ggtcggctaa ggaggccttt tccaaggcta tgggaacggg cattagcaag ctcggttttc
      301 aggatttgga agtcttgaac aatgaacgtg gggcgcctta ttttagtcag gcaccatttt
      361 caggaaagat ttggctgtct atcagccaca ccgatcagtt tgtgacagcc agtgtcattt
      421 tggaggaaaa tcatgaaagc tagggatccg gctgctaaca aagcccgaaa ggaagctgag
      481 ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc
      541 ttgaggggtt ttttgctgaa aggaggaact atatccggat atcccgcaag aggcccggca
      601 gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga
      661 tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata
      721 aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga
      781 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggt
//
