LOCUS       sequence-109225-         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of the FabI enzyme (enoyl reductase) from 
            Francisella tularensis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Hevener KE, Mehboob S, Su PC, Truong K, Boci T, Deng J, Ghassemi M, 
            Cook JL, Johnson ME
  TITLE     Discovery of a novel and potent class of F. tularensis 
            enoyl-reductase (FabI) inhibitors by molecular shape and 
            electrostatic matching.
  JOURNAL   J Med Chem. 2012 Jan 12;55(1):268-79. doi: 10.1021/jm201168g. Epub 
            2011 Dec 5.
  PUBMED    22098466
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/jm201168g";
            journalName: "J Med Chem"; date: "2012-01-12- 12"; volume: "55"; 
            issue: "1"; pages: "268-79"
COMMENT     Sequence Label: pET15b-Fab1 (Francisella tularensis)
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             41..58
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             68..85
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 tttgtttact ttaagaagga gatataccat gggcagcagc catcatcatc atcatcacag
       61 cagcggcctg gtgccgcgcg gcagccatat gggttttctg gcgggtaaga aaattctgat
      121 caccggtctg ctgtccaaca aatctattgc ctacggcatc gctaaagcca tgcaccgcga
      181 gggtgccgaa ctggcgttca cttacgtggg tcaattcaaa gaccgtgttg aaaaactgtg
      241 cgcggaattc aatccggctg cagtcctgcc gtgcgatgtt atctccgacc aggaaattaa
      301 agacctgttc gttgagctgg gtaaagtttg ggacggtctg gacgcgattg tgcactctat
      361 cgcgtttgcg ccgcgtgatc agctggaggg caatttcatt gactgtgtga cccgcgaagg
      421 cttctccatc gcacacgata tctctgcgta tagcttcgcg gccctggcca aagaaggtcg
      481 ttctatgatg aaaaaccgta acgcatccat ggtagcactg acctacatcg gtgcagagaa
      541 agcaatgcct tcctacaaca ccatgggtgt tgctaaagcg tctctggaag ctaccgtacg
      601 ttatacggct ctggcactgg gcgaagatgg tattaaagtt aacgctgtat ccgctggccc
      661 aatcaaaact ctggcggcta gcggcatcag caacttcaag aagatgctgg actataacgc
      721 gatggtttct ccgctgaaga aaaacgtgga catcatggaa gttggcaaca ccgttgcatt
      781 tctgtgtagc gatatggcta ccggcatcac tggtgaagtg gtgcacgtcg atgctggcta
      841 ccattgcgta agcatgggca acgtcctgta actcgaggat
//
