LOCUS       sequence-109226-         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of the FabI enzyme (enoyl reductase) from 
            Francisella tularensis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Hevener KE, Mehboob S, Su PC, Truong K, Boci T, Deng J, Ghassemi M, 
            Cook JL, Johnson ME
  TITLE     Discovery of a novel and potent class of F. tularensis 
            enoyl-reductase (FabI) inhibitors by molecular shape and 
            electrostatic matching.
  JOURNAL   J Med Chem. 2012 Jan 12;55(1):268-79. doi: 10.1021/jm201168g. Epub 
            2011 Dec 5.
  PUBMED    22098466
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/jm201168g";
            journalName: "J Med Chem"; date: "2012-01-12- 12"; volume: "55"; 
            issue: "1"; pages: "268-79"
COMMENT     Sequence Label: pET15b-Fab1 (Francisella tularensis)
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(810..827)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(837..854)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttgttagcag ccggatcctc gagttacagg acgttgccca tgcttacgca atggtagcca
       61 gcatcgacgt gcaccacttc accagtgatg ccggtagcca tatcgctaca cagaaatgca
      121 acggtgttgc caacttccat gatgtccacg tttttcttca gcggagaaac catcgcgtta
      181 tagtccagca tcttcttgaa gttgctgatg ccgctagccg ccagagtttt gattgggcca
      241 gcggatacag cgttaacttt aataccatct tcgcccagtg ccagagccgt ataacgtacg
      301 gtagcttcca gagacgcttt agcaacaccc atggtgttgt aggaaggcat tgctttctct
      361 gcaccgatgt aggtcagtgc taccatggat gcgttacggt ttttcatcat agaacgacct
      421 tctttggcca gggccgcgaa gctatacgca gagatatcgt gtgcgatgga gaagccttcg
      481 cgggtcacac agtcaatgaa attgccctcc agctgatcac gcggcgcaaa cgcgatagag
      541 tgcacaatcg cgtccagacc gtcccaaact ttacccagct caacgaacag gtctttaatt
      601 tcctggtcgg agataacatc gcacggcagg actgcagccg gattgaattc cgcgcacagt
      661 ttttcaacac ggtctttgaa ttgacccacg taagtgaacg ccagttcggc accctcgcgg
      721 tgcatggctt tagcgatgcc gtaggcaata gatttgttgg acagcagacc ggtgatcaga
      781 attttcttac ccgccagaaa acccatatgg ctgccgcgcg gcaccaggcc gctgctgtga
      841 tgatgatgat gatggctgct gcccatggta tatct
//
