LOCUS       sequence-109227-         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of the FabI enzyme from S. aureus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  TITLE     Johnson Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET15b-Fab1 (S. aureus)
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(802..819)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(829..846)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttgttagcag ccggatcctt atttaattgc gtggaatccg ctatctacat gaatattttc
       61 acctgtaacg ccacttgata aatcacttaa taagtaagcc gcagttttac ctacttctac
      121 ttgatcaaca ttacgtttta aaggtgcacg ctcttcgatt tctttaagaa ttgtattgaa
      181 accacccaca ccttttgcac ttaatgtacg gattggacca gctgaaattg cattaacgcg
      241 aatattatct ggacctaagt ctaatgctaa atatttaaca tttgcttcta agctcgcttt
      301 agcaacaccc atcacattat agttttgaac tgcgaattcg ccacctaaat atgttgttgc
      361 aacaatgcta ccaccttctg gcattaattt tttagcttca tgagccacaa ttgttaatga
      421 gtaagaacta atgtcttgag ctaacaagaa gccttcacgt gaagtttcag aaaagcgtcc
      481 gcgtaagtct tccatattag caaatgcgat tgaatgatat acaccatcaa tattgccaac
      541 atctttacca atttgctcaa aaccattaat aacctcttca tcgctttgaa catcaatttg
      601 atataagtgc gcttctggtt gatttaattg ttctaataat ttttcaagct ctttacggct
      661 acgttcttta cggtaagtaa atactaattt agcacctaat tgatctaaaa ctttagcgac
      721 accaaaagca atactacgct tattagcgat tcccatgatg acatatgttt tgttttcaag
      781 atttaacatc tcgagcatat ggctgccgcg cggcaccagg ccgctgctgt gatgatgatg
      841 atgatggctg ctgcccatg
//
