LOCUS       sequence-109228-         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of the FabI enzyme from S. aureus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  TITLE     Johnson Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET15b-Fab1 (S. aureus)
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             30..47
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             57..74
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 taagaaggag atataccatg ggcagcagcc atcatcatca tcatcacagc agcggcctgg
       61 tgccgcgcgg cagccatatg ctcgagatgt taaatcttga aaacaaaaca tatgtcatca
      121 tgggaatcgc taataagcgt agtattgctt ttggtgtcgc taaagtttta gatcaattag
      181 gtgctaaatt agtatttact taccgtaaag aacgtagccg taaagagctt gaaaaattat
      241 tagaacaatt aaatcaacca gaagcgcact tatatcaaat tgatgttcaa agcgatgaag
      301 aggttattaa tggttttgag caaattggta aagatgttgg caatattgat ggtgtatatc
      361 attcaatcgc atttgctaat atggaagact tacgcggacg cttttctgaa acttcacgtg
      421 aaggcttctt gttagctcaa gacattagtt cttactcatt aacaattgtg gctcatgaag
      481 ctaaaaaatt aatgccagaa ggtggtagca ttgttgcaac aacatattta ggtggcgaat
      541 tcgcagttca aaactataat gtgatgggtg ttgctaaagc gagcttagaa gcaaatgtta
      601 aatatttagc attagactta ggtccagata atattcgcgt taatgcaatt tcagctggtc
      661 caatccgtac attaagtgca aaaggtgtgg gtggtttcaa tacaattctt aaagaaatcg
      721 aagagcgtgc acctttaaaa cgtaatgttg atcaagtaga agtaggtaaa actgcggctt
      781 acttattaag tgatttatca agtggcgtta
//
