LOCUS       sequence-109230-         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  inducible expression of SARS 3CLpro protease.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Lee H, Mittal A, Patel K, Gatuz JL, Truong L, Torres J, Mulhearn DC,
            Johnson ME
  TITLE     Identification of novel drug scaffolds for inhibition of SARS-CoV 
            3-Chymotrypsin-like protease using virtual and high-throughput 
            screenings.
  JOURNAL   Bioorg Med Chem. 2014 Jan 1;22(1):167-77. doi: 
            10.1016/j.bmc.2013.11.041. Epub 2013 Dec 1.
  PUBMED    24332657
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bmc.2013.11.041"; journalName: "Bioorg Med Chem"; date: 
            "2014-01-1- 1"; volume: "22"; issue: "1"; pages: "167-77"
COMMENT     Sequence Label: pGEX-6P-1 SARS-CoV 3CLpro
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(59..76)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cgaggcagat cgtcagtcag tcacgatgcg gccgctcgag tcgacccggg aattcttagt
       61 gatgatgatg gtgatgtggg ccctggaacg tgacaccgct gcactgacgt actacgtcga
      121 acggggtaaa ttcgtcctcc agaatggtgc tacccaggat agtacgaccg ttcatgccgt
      181 tctgcagcag ttctttcagc gcagcacaca tgtccagcac tgcaataccg gtctgcgcgg
      241 acagcgggcc caggatgtct acgtgatctt gggtcagcgg ctcgtagttg tatttcatag
      301 ctaccaggtt gaagtcgttc agggtagtgg tgaaacggtt caggaaccag cggtcgccgt
      361 tgataacagc tgcgtacagc caggccagaa cgttcagagt gatggtggtg tccgtaccag
      421 cggcctgtgc ggtctgacgg tccacgaacg gaccgtagaa cttaccttcc agatcggtgc
      481 cggcgtgaac acccgtcggc agttccatgt ggtgcatgta acagaagctc acacaatcgt
      541 aatcgatatt aaagccaacg gagccgcagg agccgttcag aaaagaacct ttgatagtgt
      601 gattcggacg catcgcacac tggtacacgc cagacggaga accgttatag caagccagta
      661 cgctgaacgt ctggccaggc tggatacgca cgaacttata tttaggggtt ttcgggttag
      721 aagtatccac tttcagacgc agcaggcagt tctgcatgga gtgaccgata acgcgcagtt
      781 gaacgttacc cgcctgaacc agaaaggagt ggttagattt gcgaatcagc agatc
//
