LOCUS       sequence-109235-         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  inducible expression of SARS PLpro protease.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Lee H, Cao S, Hevener KE, Truong L, Gatuz JL, Patel K, Ghosh AK, 
            Johnson ME
  TITLE     Synergistic inhibitor binding to the papain-like protease of human 
            SARS coronavirus: mechanistic and inhibitor design implications.
  JOURNAL   ChemMedChem. 2013 Aug;8(8):1361-72. doi: 10.1002/cmdc.201300134. 
            Epub 2013 Jun 20.
  PUBMED    23788528
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/cmdc.201300134"; journalName: "ChemMedChem"; date: 
            "2013-08"; volume: "8"; issue: "8"; pages: "1361-72"
COMMENT     Sequence Label: pET15b SARS-CoV PLpro
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(22..39)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(40..63)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 agcagccgga tcctcgagtt aatgatggtg gtgatggtgt ggaccctgaa acagaacttc
       61 cagcttgata gtagtggtgt agctggtctc tttgtagaag acgtccgtta ccgggccttt
      121 gtattcagac attttggtca ggtgggcacc atcgatacgg tacagggtct ccttggccgt
      181 gatgtgagtg tagtgaccgc actggtagtt accagtatac tcgtttgcgc acagaaaggt
      241 gccttgctgc agtttatatt cggcaggtgg agcagacatc ataacgaagg aggattcctg
      301 ctgtaccagg tactgagttg catcacgacc acaaacgcac gggatagaaa cgcccgtttt
      361 caggttgtca taggacagag tacccatata catcactgct tcaacgccgg tcagggtggt
      421 agttttctgg ccgcaatgtt tacagaccac gttcagtacg cgtttagcgg attccagatt
      481 agcatgttgc agcaggtggg tcatggtttc acgaacatcg cccagttcgc ccacggtctt
      541 gttggaataa gccaggatca gagcacagaa gtttgccgca tcacccgcgc gtgcacggta
      601 atacgcttcc tgcagagccg gcgcgttgaa cttaacctcc agctgttgca gggccagcag
      661 aacgctggac aggtagcagt tgttgtctgc ccatttaatg ctggtcagac cgccaacctg
      721 cgggaatttc cattttttag tgtggttcag cgcagacatg taacgaccca ggaaagattc
      781 gtccagggtg tggtagtatt cgaacgcttc agagcgcagg gtatcgtcgc tcggc
//
