LOCUS       sequence-109237-         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  inducible dual expression of NS3 protease along with its cofactor 
            NS4A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Lee H, Zhu T, Patel K, Zhang YY, Truong L, Hevener KE, Gatuz JL, 
            Subramanya G, Jeong HY, Uprichard SL, Johnson ME
  TITLE     High-throughput screening (HTS) and hit validation to identify small
            molecule inhibitors with activity against NS3/4A proteases from 
            multiple hepatitis C virus genotypes.
  JOURNAL   PLoS One. 2013 Oct 9;8(10):e75144. doi: 
            10.1371/journal.pone.0075144. eCollection 2013.
  PUBMED    24130685
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-9"; volume: "8(10):e75144. doi"; pages: " 
            10.1371/journal.pone.0075144. eCollection 2013"
COMMENT     Sequence Label: pETDuet-1 Hepatitis C Virus NS3/NS4A
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    71..95
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             110..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     promoter        388..406
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    407..431
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             477..494
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gcccgatgcg tccggcgtag aggatcgaga tcgatctcga tcccgcgaaa ttaatacgac
       61 tcactatagg ggaattgtga gcggataaca attcccctct agaaataatt ttgtttaact
      121 ttaagaagga gatataccat gggtagcact tgggtactgg taggtggtgt gctggctgcg
      181 ctggcggctt actgcctgac tacgggttct gttgtaatcg taggtcgcat catcctgtcc
      241 ggccgtccgg caattgtccc agatcgtgaa ctgctgtatc aggaattcga tgaaatggaa
      301 gaaggtaaga agaaataagc ttgcggccgc ataatgctta agtcgaacag aaagtaatcg
      361 tattgtacac ggccgcataa tcgaaattaa tacgactcac tataggggaa ttgtgagcgg
      421 ataacaattc cccatcttag tatattagtt aagtataaga aggagatata catatgcacc
      481 accatcatca ccatgcaccg attaccgcgt attctcagca aacgcgcggt ctgctgggct
      541 gtattatcac gtccctgact ggtcgtgaca aaaaccaggt ggaaggcgaa gtgcaggtcg
      601 tttctactgc aacccagtcc ttcctggcca cctgcgtaaa cggcgtctgc tggaccgttt
      661 accacggtgc gggcagcaaa accctggccg cgccgaaagg cccaattact cagatgtata
      721 ccaacgtaga tcaggatctg gttggttggc cgaaaccgcc tggtgcacgc tccctgaccc
      781 cgtgtacctg cggctcttcc gacctgtatc tggtcacgcg tcatgcggat gtaatcccgg
//
