LOCUS       sequence-109238-         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  inducible dual expression of NS3 protease along with its cofactor 
            NS4A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Lee H, Zhu T, Patel K, Zhang YY, Truong L, Hevener KE, Gatuz JL, 
            Subramanya G, Jeong HY, Uprichard SL, Johnson ME
  TITLE     High-throughput screening (HTS) and hit validation to identify small
            molecule inhibitors with activity against NS3/4A proteases from 
            multiple hepatitis C virus genotypes.
  JOURNAL   PLoS One. 2013 Oct 9;8(10):e75144. doi: 
            10.1371/journal.pone.0075144. eCollection 2013.
  PUBMED    24130685
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-9"; volume: "8(10):e75144. doi"; pages: " 
            10.1371/journal.pone.0075144. eCollection 2013"
COMMENT     Sequence Label: pETDuet-1 Hepatitis C Virus NS3/NS4A
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(8..52)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 tagtagacga gtccatgtgc tggcgttcaa atttcgcagc agcggtttct ttaccagact
       61 cgagttaagt aacaacctcc aggtcagcgc tcatgcaagc catgatgtac ttggtgatag
      121 gatgcgtcag agtaacctcg ttctggacag cacccagacg atacagcagc ggggtcgggc
      181 cgtgcagggt cggtttcaga cggatcagac atttccacat ctggtcccag gacggcggtg
      241 gtgcctgcgc acgagcacat acagtagcct gatatgcaac caggtacgga aagttatcgc
      301 cagcttgttt agtctgagac aggaagtgcg catcgatgtg agtcaggccg gtaaaaacgg
      361 attcccagaa ttccaggtgg tcctgacaca ctggcagacc cggggtgttc aggtaggcac
      421 gcagacgaac gctagtctca gcaggagtca gttcgtacca cgcgcagcca gcgtcgtagc
      481 actcgcacag aacagaggag tcgaacatgc cgctcggacg ttcacctgga gtgacaaaac
      541 ggtaaatgcc acgacggccg cgaccggtac ggccacgacg ttgagagcgg ctaacagcgt
      601 cctgcggcac agtagtggtt tcgatagtaa aggtcgggtc cagagagaaa tccacagtct
      661 gggttacgca ggtgttgcta tcgatcacag agtcgaagtc accggtgtag ccggtcatca
      721 gtgcgtccgt agcaactacg acgacgtcac cgatggtcgg aataacagac acgtccagac
      781 cacggtagta cgccactgcg ttgataccca gaccgctcag tttagccgcc agttcgtcac
      841 attt
//
