LOCUS       sequence-109249-         701 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT 
            core (aa 1048-1664; mutation 1645/1646 RR/EE) driven by CMV 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Hilton IB, D'Ippolito AM, Vockley CM, Thakore PI, Crawford GE, Reddy
            TE, Gersbach CA
  TITLE     Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates
            genes from promoters and enhancers.
  JOURNAL   Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 
            2015 Apr 6.
  PUBMED    25849900
REFERENCE   2  (bases 1 to 701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3199"; 
            journalName: "Nat Biotechnol"; date: "2015-05"; volume: "33"; issue:
            "5"; pages: "510-7"
COMMENT     Sequence Label: pcDNA-dCas9-p300 Core (1645/1646 RR/EE)
FEATURES             Location/Qualifiers
     source          1..701
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cgcaaagttg ggcgcgccat tttcaaacca gaagaactac gacaggcact gatgccaact
       61 ttggaggcac tttaccgtca ggatccagaa tcccttccct ttcgtcaacc tgtggaccct
      121 cagcttttag gaatccctga ttactttgat attgtgaaga gccccatgga tctttctacc
      181 attaagagga agttagacac tggacagtat caggagccct ggcagtatgt cgatgatatt
      241 tggcttatgt tcaataatgc ctggttatat aaccggaaaa catcacgggt atacaaatac
      301 tgctccaagc tctctgaggt ctttgaacaa gaaattgacc cagtgatgca aagccttgga
      361 tactgttgtg gcagaaagtt ggagttctct ccacagacac tgtgttgcta cggcaaacag
      421 ttgtgcacaa tacctcgtga tgccacttat tacagttacc agaacaggta tcatttctgt
      481 gagaagtgtt tcaatgagat ccaaggggag agcgtttctt tgggggatga cccttcccag
      541 cctcaaacta caataaataa agaacaattt tccaagagaa aaaatgacac actggatcct
      601 gaactgtttg ttgaatgtac agagtgcgga agaaagatgc atcagatctg tgtccttcac
      661 catgagatca tctggcctgc tggattcgtc tgtgatggct g
//
