LOCUS       sequence-109251-         649 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT 
            core (aa 1048-1664; inactivating mutation D1399Y) driven by CMV 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Hilton IB, D'Ippolito AM, Vockley CM, Thakore PI, Crawford GE, Reddy
            TE, Gersbach CA
  TITLE     Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates
            genes from promoters and enhancers.
  JOURNAL   Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 
            2015 Apr 6.
  PUBMED    25849900
REFERENCE   2  (bases 1 to 649)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3199"; 
            journalName: "Nat Biotechnol"; date: "2015-05"; volume: "33"; issue:
            "5"; pages: "510-7"
COMMENT     Sequence Label: pcDNA-dCas9-p300 Core (D1399Y)
FEATURES             Location/Qualifiers
     source          1..649
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      632..641
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 catgcatgtt caagagtatg gctctgactg ccctccaccc aaccagagga gagtatacat
       61 atcttacctc tatagtgttc atttcttccg tcctaaatgc ttgaggactg cagtctatca
      121 tgaaatccta attggatatt tagaatatgt caagaaatta ggttacacaa cagggcatat
      181 ttgggcatgt ccaccaagtg agggagatga ttatatcttc cattgccatc ctcctgacca
      241 gaagataccc aagcccaagc gactgcagga atggtacaaa aaaatgcttg acaaggctgt
      301 atcagagcgt attgtccatg actacaagga tatttttaaa caagctactg aagatagatt
      361 aacaagtgca aaggaattgc cttatttcga gggtgatttc tggcccaatg ttctggaaga
      421 aagcattaag gaactggaac aggaggaaga agagagaaaa cgagaggaaa acaccagcaa
      481 tgaaagcaca gatgtgacca agggagacag caaaaatgct aaaaagaaga ataataagaa
      541 aaccagcaaa aataagagca gcctgagtag gggcaacaag aagaaacccg ggatgcccaa
      601 tgtatctaac gacctctcac agaaactata tgccaccatg gagaagcat
//
