LOCUS       sequence-109252-         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes N. meningiditis dCas9 with c-terminal fusion of human p300 
            HAT core (aa 1048-1664) driven by CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Hilton IB, D'Ippolito AM, Vockley CM, Thakore PI, Crawford GE, Reddy
            TE, Gersbach CA
  TITLE     Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates
            genes from promoters and enhancers.
  JOURNAL   Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 
            2015 Apr 6.
  PUBMED    25849900
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3199"; 
            journalName: "Nat Biotechnol"; date: "2015-05"; volume: "33"; issue:
            "5"; pages: "510-7"
COMMENT     Sequence Label: pcDNA3.3-Nm-dCas9-p300 Core
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             23..43
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tcctgtgcgc agcagggctg accccaagaa gaagaggaag gtggaggcca gcgggcgcgc
       61 cattttcaaa ccagaagaac tacgacaggc actgatgcca actttggagg cactttaccg
      121 tcaggatcca gaatcccttc cctttcgtca acctgtggac cctcagcttt taggaatccc
      181 tgattacttt gatattgtga agagccccat ggatctttct accattaaga ggaagttaga
      241 cactggacag tatcaggagc cctggcagta tgtcgatgat atttggctta tgttcaataa
      301 tgcctggtta tataaccgga aaacatcacg ggtatacaaa tactgctcca agctctctga
      361 ggtctttgaa caagaaattg acccagtgat gcaaagcctt ggatactgtt gtggcagaaa
      421 gttggagttc tctccacaga cactgtgttg ctacggcaaa cagttgtgca caatacctcg
      481 tgatgccact tattacagtt accagaacag gtatcatttc tgtgagaagt gtttcaatga
      541 gatccaaggg gagagcgttt ctttggggga tgacccttcc cagcctcaaa ctacaataaa
      601 taaagaacaa ttttccaaga gaaaaaatga cacactggat cctgaactgt ttgttgaatg
      661 tacagagtgc ggaagaaaga tgcatcagat ctgtgtcctt caccatgaga tcatctggcc
      721 tgctggattc gtctgtgatg gctg
//
