LOCUS       sequence-109256-         636 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT 
            core (aa 1048-1664; inactivating mutations 
            H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A) driven by CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Hilton IB, D'Ippolito AM, Vockley CM, Thakore PI, Crawford GE, Reddy
            TE, Gersbach CA
  TITLE     Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates
            genes from promoters and enhancers.
  JOURNAL   Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 
            2015 Apr 6.
  PUBMED    25849900
REFERENCE   2  (bases 1 to 636)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3199"; 
            journalName: "Nat Biotechnol"; date: "2015-05"; volume: "33"; issue:
            "5"; pages: "510-7"
COMMENT     Sequence Label: pcDNA-dCas9-p300 Core
            (H1415A/E1423A/Y1424A/L1...
FEATURES             Location/Qualifiers
     source          1..636
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      619..628
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gagtatggct ctgactgccc tccacccaac cagaggagag tatacatatc ttacctcgat
       61 agtgttcatt tcttccgtcc taaatgcttg aggactgcag tctatgccga aatcctaatt
      121 ggatatttag cggccgtcaa gaaatccgga gccacaacag gggccatttg ggcatgtcca
      181 ccaagtgagg gagatgatta tatcttccat tgccatcctc ctgaccagaa gatacccaag
      241 cccaagcgac tgcaggaatg gtacaaaaaa atgcttgaca aggctgtatc agagcgtatt
      301 gtccatgact acaaggatat ttttaaacaa gctactgaag atagattaac aagtgcaaag
      361 gaattgcctt atttcgaggg tgatttctgg cccaatgttc tggaagaaag cattaaggaa
      421 ctggaacagg aggaagaaga gagaaaacga gaggaaaaca ccagcaatga aagcacagat
      481 gtgaccaagg gagacagcaa aaatgctaaa aagaagaata ataagaaaac cagcaaaaat
      541 aagagcagcc tgagtagggg caacaagaag aaacccggga tgcccaatgt atctaacgac
      601 ctctcacaga aactatatgc caccatggag aagcat
//
