LOCUS       sequence-109257-         702 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT 
            core (aa 1048-1664; inactivating mutations 
            H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A) driven by CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Hilton IB, D'Ippolito AM, Vockley CM, Thakore PI, Crawford GE, Reddy
            TE, Gersbach CA
  TITLE     Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates
            genes from promoters and enhancers.
  JOURNAL   Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 
            2015 Apr 6.
  PUBMED    25849900
REFERENCE   2  (bases 1 to 702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3199"; 
            journalName: "Nat Biotechnol"; date: "2015-05"; volume: "33"; issue:
            "5"; pages: "510-7"
COMMENT     Sequence Label: pcDNA-dCas9-p300 Core
            (H1415A/E1423A/Y1424A/L1...
FEATURES             Location/Qualifiers
     source          1..702
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gttgggcgcg ccattttcaa accagaagaa ctacgacagg cactgatgcc aactttggag
       61 gcactttacc gtcaggatcc agaatccctt ccctttcgtc aacctgtgga ccctcagctt
      121 ttaggaatcc ctgattactt tgatattgtg aagagcccca tggatctttc taccattaag
      181 aggaagttag acactggaca gtatcaggag ccctggcagt atgtcgatga tatttggctt
      241 atgttcaata atgcctggtt atataaccgg aaaacatcac gggtatacaa atactgctcc
      301 aagctctctg aggtctttga acaagaaatt gacccagtga tgcaaagcct tggatactgt
      361 tgtggcagaa agttggagtt ctctccacag acactgtgtt gctacggcaa acagttgtgc
      421 acaatacctc gtgatgccac ttattacagt taccagaaca ggtatcattt ctgtgagaag
      481 tgtttcaatg agatccaagg ggagagcgtt tctttggggg atgacccttc ccagcctcaa
      541 actacaataa ataaagaaca attttccaag agaaaaaatg acacactgga tcctgaactg
      601 tttgttgaat gtacagagtg cggaagaaag atgcatcaga tctgtgtcct tcaccatgag
      661 atcatctggc ctgctggatt cgtctgtgat ggctgtttaa ag
//
