LOCUS       sequence-109264-         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of alpha-actinin with internal sstFRET inserted to 
            measure cytoskeletal stress in vivo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Meng F, Sachs F
  TITLE     Visualizing dynamic cytoplasmic forces with a compliance-matched 
            FRET sensor.
  JOURNAL   J Cell Sci. 2011 Jan 15;124(Pt 2):261-9. doi: 10.1242/jcs.071928. 
            Epub 2010 Dec 20.
  PUBMED    21172803
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2011-01-15"; volume: "124(Pt 2):261-9. doi"; pages: " 
            10.1242/jcs.071928. Epub 2010 Dec 20"
COMMENT     Sequence Label: PEG-Actinin-M-sstFRET
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     96..117
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(490..741)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(532..553)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc
       61 gccctcgccg gacacgctga acttgtggcc gtttacgtcg ccgtccagct cgaccaggat
      121 gggcaccacc ccggtgaaca gctcctcgcc cttgctcacc atggaattca cgcctccctt
      181 tgccttgcgc tgatctgcac gtttccccag gctgctccag gcctggttta actctgtgca
      241 cttttcctgc aggtcttctg ctgactcggg atgggactgg atcaggcgct ctgctgtttc
      301 accaagggag tttaccttgt caccgagagc cgcaaggtcc ctctcgaagc cctcatgctt
      361 gcgttgcagg gcctggacac tggcgagatc atgtccataa ttgtctgtgt ttagagcttg
      421 attcttctct tcaatccatt ctttggtttc atcagcatct ctgtggaagc ctccagatct
      481 gagtccggac ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc
      541 cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg ctcagtttgg actggtagct
      601 caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt tctgctggta
      661 gtggtcggcg agctgcacgc cgccgtcctc gatgttgtgg cggatcttga agttggcctt
      721 gatgccgttc ttctgcttgt cggcggtgat atagacgttg tggctgttgt agttgtactc
      781 cagcttgtg
//
