LOCUS       sequence-109269-         746 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of EGFP-TEV-S-tag Nuf2 for protein 
            localization and affinity purification (LAP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 746)
  TITLE     Cheeseman Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pIC162
FEATURES             Location/Qualifiers
     source          1..746
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..67
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             80..124
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 gtccggaggg gatcggtgga gctccaccgg tggcggccgc tctagagaga atctttattt
       61 tcagggcgcc gccaaattca aagaaaccgc tgctgctaaa ttcgaacgcc agcacatgga
      121 cagcggaggt ggaggtacta gtctcgagat ggaaactttg tctttcccca gatataatgt
      181 agctgagatt gtgattcata ttcgcaataa gatcttaaca ggagctgatg gtaaaaacct
      241 caccaagaat gatctttatc caaatccaaa gcctgaagtc ttgcacatga tctacatgag
      301 agccttacaa atagtctatg gaattcgact ggaacatttt tacatgatgc cagtgaactc
      361 tgaagtcatg tatccacatt taatggaagg cttcttacca ttcagcaatt tagttactca
      421 tctggactca tttttgccta tctgccgggt gaatgacttt gagactgctg atattctatg
      481 tccaaaagca aaacggacaa gtcggttttt aagtggcatt atcaacttta ttcacttcag
      541 agaagcatgc cgtgaaacgt atatggaatt tctttggcaa tataaatcct ctgcggacaa
      601 aatgcaacag ttaaacgccg cacaccagga ggcattaatg aaactggaga gacttgattc
      661 tgttccagtt gaagagcaag aagagttcaa gcagctttca gatggaattc aggagctaca
      721 acaatcacta aatcaggatt ttcatc
//
