LOCUS       sequence-109270-         699 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral transfer plasmid. Reporter for GLUT4 
            translocation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Lim CY, Bi X, Wu D, Kim JB, Gunning PW, Hong W, Han W
  TITLE     Tropomodulin3 is a novel Akt2 effector regulating insulin-stimulated
            GLUT4 exocytosis through cortical actin remodeling.
  JOURNAL   Nat Commun. 2015 Jan 9;6:5951. doi: 10.1038/ncomms6951.
  PUBMED    25575350
REFERENCE   2  (bases 1 to 699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-01-9"; volume: "6:5951. doi"; pages: " 
            10.1038/ncomms6951"
COMMENT     Sequence Label: pLenti-myc-GLUT4-mCherry
FEATURES             Location/Qualifiers
     source          1..699
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(251..274)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(449..468)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gtaatccaga ggttgattat cgataagctt gatatcgaat tgttaacaac aacaattgca
       61 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
      121 tctacaaatg tggtatggct gattatgatc tagagtcgcg gccgctttac ttgtacgtac
      181 agctcatcca tgccgccggt ggagtggcgg ccctcggcgc gctcgtactg ttccacgatg
      241 gtgtagtcct cgttgtggga ggtgatgtcc agcttgatgt tgacgttgta ggcgccgggc
      301 agctgcacgg gcttcttggc cttgtaggtg gtcttgacct cggcgtcgta gtggccgccg
      361 tccttcagct tcagcctctg cttgatctcg cccttcaggg cgccgtcctc ggggtacatc
      421 cgctcggagg aggcctccca gcccatggtc ttcttctgca ttacggggcc gtcggagggg
      481 aagttggtgc cgcgcagctt caccttgtag atgaactcgc cgtcctgcag ggaggagtcc
      541 tgggtcacgg tcaccacgcc gccgtcctcg aagttcatca cgcgctccca cttgaagccc
      601 tcggggaagg acagcttcaa gtagtcgggg atgtcggcgg ggtgcttcac gtaggccttg
      661 gagccgtaca tgaactgagg ggacaggatg tcccaggcg
//
