LOCUS       sequence-109271-         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral transfer plasmid. Reporter for GLUT4 
            translocation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Lim CY, Bi X, Wu D, Kim JB, Gunning PW, Hong W, Han W
  TITLE     Tropomodulin3 is a novel Akt2 effector regulating insulin-stimulated
            GLUT4 exocytosis through cortical actin remodeling.
  JOURNAL   Nat Commun. 2015 Jan 9;6:5951. doi: 10.1038/ncomms6951.
  PUBMED    25575350
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-01-9"; volume: "6:5951. doi"; pages: " 
            10.1038/ncomms6951"
COMMENT     Sequence Label: pLenti-myc-GLUT4-mCherry
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             299..310
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      395..404
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tcgatctcga actcgtggcc gttcacggag ccctccatgt gcaccttgaa gcgcatgaac
       61 tccttgatga tggccatgtt gtcctcctcg cccttgctca ccagggttgt gaattccggt
      121 ggatccccgt cattctcatc tggccctaag tattcaagtt ctgtactggg tttcacctcc
      181 tgctctaaaa gggaaggtgt ccgtcggaag gcagctgaga tctggtcaaa cgtccggcct
      241 ctggtttcag gcacttttag gaaggtgaag atgaagaagc caagcaggag gacggcaaat
      301 agaaggaaga cgtaaggacc catagcatcc gcaacatact ggaaacccat gccgacaatg
      361 aagttacagg tccagttgga gaaaccagcg acagccatgg cggctgggcg ggggccctgg
      421 ctgaagagct ctgccacaat gaaccaggga atggggccag ggccaatctc aaagaaggcc
      481 acaaagccaa atatggccac gatggagaca tagctcatgg ctggaacccg ttccagcagc
      541 agcagagcca cggtcatcaa gatggcacag ccacacatgc cggccaggcc caacagatgg
      601 agtgtccgtc gtccagctcg ttctactaag agcaccgaga ccaacgtgaa gaccgtattg
      661 accacaccag ctcctatggt ggcgtaggct ggctgtccca ccccagccga ctcgaagatg
      721 ctggttgaat agtagaaaac agcattgatg cctgagagct gttggctcag ctgcagcacc
      781 actgcgatga tcaga
//
