LOCUS       sequence-109274-         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination vector with zebrafish aA-crystallin promoter 
            driving mCherry GFP in lens.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Berger J, Currie PD
  TITLE     503unc, a small and muscle-specific zebrafish promoter.
  JOURNAL   Genesis. 2013 Jun;51(6):443-7. doi: 10.1002/dvg.22385. Epub 2013 Mar
            26.
  PUBMED    23444339
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/dvg.22385";
            journalName: "Genesis"; date: "2013-06"; volume: "51"; issue: "6"; 
            pages: "443-7"
COMMENT     Sequence Label: pDESTtol2pACrymCherry
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    86..207
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(97..116)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     151..170
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(410..433)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(608..627)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tattagtctt gatagagagg ctgcaaatag caggaaacgt gagcagagac tccctggtgt
       61 ctgaaacaca ggcccattta cgccttaaga tacattgatg agtttggaca aaccacaact
      121 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
      181 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
      241 gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg
      301 gctgattatg atctagagtc gcggccgctt tacttgtaca gctcgtccat gccgccggtg
      361 gagtggtggc cctcggcgcg ctcgtactgt tccacgatgg tgtagtcctc gttgtgggag
      421 gtgatgtcca gcttgatgtt gacgttgtag gcgccgggca gctgcacggg cttcttggcc
      481 ttgtaggtgg tcttgacctc ggcgtcgtag tggccgccgt ccttcagctt cagcctctgc
      541 ttgatctcgc ccttcagggc gccgtcctcg gggtacatcc gctcggagga ggcctcccag
      601 cccatggtct tcttctgcat tacggggccg tcggagggga agttggtgcc gcgcagcttc
      661 accttgtaga tgaactcgcc gtcctgcagg gaggagtcct gggtcacggt caccacgccg
      721 ccgtcctcga agttcatcac gcgctcccac ttgaagccct cggggaagga cagcttcaag
      781 tagtcgggga tgtcggcggg gtgcttcacg taggccttgg agccgtacat gaactgaggg
      841 gacaggatgt ccc
//
