LOCUS       sequence-109280-         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination vector for Gateway based on vector #394 from the 
            Tol2Kit, containing a gRNA scaffold under the control of a zebrafish
            U6 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Ablain J, Durand EM, Yang S, Zhou Y, Zon LI
  TITLE     A CRISPR/Cas9 Vector System for Tissue-Specific Gene Disruption in 
            Zebrafish.
  JOURNAL   Dev Cell. 2015 Mar 4. pii: S1534-5807(15)00075-1. doi: 
            10.1016/j.devcel.2015.01.032.
  PUBMED    25752963
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2015 Mar 4. pii: S1534-5807(15)00075-1. doi: 
            10.1016/j.devcel.2015.01.032."
COMMENT     Sequence Label: pDestTol2pA2-U6:gRNA
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     14..30
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14..30
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_feature    join(64..75,76..263)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     polyA_signal    702..783
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(739..758)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 cggtggcggc cgctctagaa ctagtggatc tgctgggctt gctgaaggta gggggtcaag
       61 aaccagaggt gtaaagtact tgagtaattt tacttgatta ctgtacttaa gtattatttt
      121 tggggatttt tactttactt gagtacaatt aaaaatcaat acttttactt ttacttaatt
      181 acattttttt agaaaaaaaa gtacttttta ctccttacaa ttttatttac agtcaaaaag
      241 tacttatttt ttggagatca cttcattcta ttttcccttg ctattaccaa accaattgaa
      301 ttgcgctgat gcccagttta atttaaatgt tatttattct gcctatgaaa atcgttttca
      361 cattatatga aattggtcag acatgttcat tggtcctttg gaagtgacgt catgtcacat
      421 ctattaccac aatgcacagc accttgacct ggaaattagg gaaattataa cagtcaatca
      481 gtggaagaaa atggaggaag tatgtgattc atcagcagct gcgagcagca cagtccaaaa
      541 tcagccacag gatcaagagc acccgtggcc gtatcttcgc agatctgatc tagaggatca
      601 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
      661 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
      721 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
      781 tgcattcta
//
