LOCUS       sequence-109281-         679 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Arabinose inducible lambda red and anhydrotetracycline inducible 
            sgRNA expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Reisch CR, Prather KL
  TITLE     The no-SCAR (Scarless Cas9 Assisted Recombineering) system for 
            genome editing in Escherichia coli.
  JOURNAL   Sci Rep. 2015 Oct 14;5:15096. doi: 10.1038/srep15096.
  PUBMED    26463009
REFERENCE   2  (bases 1 to 679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-10-14"; volume: "5:15096. doi"; pages: " 
            10.1038/srep15096"
COMMENT     Sequence Label: pKDsgRNA-ack
FEATURES             Location/Qualifiers
     source          1..679
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(13..30)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(46..75)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_RNA        complement(96..171)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    202..220
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    227..245
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 cgtttaaact caatgatgat gatgatgatg gtcgacggcg ctattcagat cctcttctga
       61 gatgagtttt tgttcgggcc caagcttcaa aaaaagcacc gactcggtgc cactttttca
      121 agttgataac ggactagcct tattttaact tgctatttct agctctaaaa caaagccgct
      181 tcctgtttat tgtgctcagt atctctatca ctgataggga tgtcaatctc tatcactgat
      241 agggaagaat tccagaaatc atccttagcg aaagctgata atctcatgac caaaatccct
      301 taacgtgagt tttcgttcca ctgagcgtca gaccccgttg atgataccgc tgccttactg
      361 ggtgcattag ccagtctgaa tgacctgtca cgggataatc cgaagtggtc agactggaaa
      421 atcagagggc aggaactgct gaacagcaaa aagtcagata gcaccacata gcagacccgc
      481 cataaaacgc cctgagaagc ccgtgacggg cttttcttgt attatgggta gtttccttgc
      541 atgaatccat aaaaggcgcc tgtagtgcca tttaccccca ttcactgcca gagccgtgag
      601 cgcagcgaac tgaatgtcac gaaaaagaca gcgactcagg tgcctgatgg tcggagacaa
      661 aaggaatatt cagcgattt
//
