LOCUS       sequence-109286-         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination vector with zebrafish aA-crystallin promoter 
            driving GFP in lens.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Berger J, Currie PD
  TITLE     503unc, a small and muscle-specific zebrafish promoter.
  JOURNAL   Genesis. 2013 Jun;51(6):443-7. doi: 10.1002/dvg.22385. Epub 2013 Mar
            26.
  PUBMED    23444339
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/dvg.22385";
            journalName: "Genesis"; date: "2013-06"; volume: "51"; issue: "6"; 
            pages: "443-7"
COMMENT     Sequence Label: pDestTol2pACryGFP
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(79..85,86..103,104..109))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(134..257)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(265..281)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    297..431
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(321..340)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     375..394
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgacggat
       61 cctaactcaa aatccacaca ttatacgagc cggaagcata aagtgtaaag cctggggtgc
      121 ctaatgcggc cgccatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt
      181 tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt ctcgttcaac
      241 tttattatac atagttgata attcactggc cgtcgtttta cggtaccatc gatgatgatc
      301 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
      361 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
      421 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt
      481 gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatcctc
      541 tagatcagat ctgcgaagat acggccacgg gtgctcttga tcctgtggct gattttggac
      601 tgtgctgctc gcagctgctg atgaatcaca tacttcctcc attttcttcc actgattgac
      661 tgttataatt tccctaattt ccaggtcaag gtgctgtgca ttgtggtaat agatgtgaca
      721 tgacgtcact tccaaaggac caatgaacat gtctgaccaa tttcatataa tgtgaaaacg
      781 attttcatag gcagaataaa taacatttaa attaaactgg gcatcagcgc aattcaattg
      841 gtttggtaat agcaagggaa aatagaatga agtgatctcc aaaaaataa
//
