LOCUS       sequence-109295-         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination vector with zebrafish aA-crystallin promoter 
            driving GFP in lens.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Berger J, Currie PD
  TITLE     503unc, a small and muscle-specific zebrafish promoter.
  JOURNAL   Genesis. 2013 Jun;51(6):443-7. doi: 10.1002/dvg.22385. Epub 2013 Mar
            26.
  PUBMED    23444339
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/dvg.22385";
            journalName: "Genesis"; date: "2013-06"; volume: "51"; issue: "6"; 
            pages: "443-7"
COMMENT     Sequence Label: pDestTol2pACryGFP
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    83..204
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(94..113)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     148..167
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(372..393)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     721..740
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ttagtcttga tagagaggct gcaaatagca ggaaacgtga gcagagactc cctggtgtct
       61 gaaacacagg ccagattggc cttaagatac attgatgagt ttggacaaac cacaactaga
      121 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
      181 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
      241 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct
      301 gattatgatc tagagtcgcg gccgctttac ttgtacagct cgtccatgcc gagagtgatc
      361 ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg
      421 ctcagggcgg actgggtgct caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg
      481 atgggggtgt tctgctggta gtggtcggcg agctgcacgc tgccgtcctc gatgttgtgg
      541 cggatcttga agttcacctt gatgccgttc ttctgcttgt cggccatgat atagacgttg
      601 tggctgttgt agttgtactc cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg
      661 atgcccttca gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg
      721 gtcttgtagt tgccgtcgtc cttgaagaag atggtgcgct cctggacgta gccttcgggc
      781 atggcggact tgaagaagtc gtgctgcttc atgtggtcgg ggtagcggct gaagcactgc
      841 acgccgtagg tcaggg
//
