LOCUS       sequence-109296-         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination vector with zebrafish aA-crystallin promoter 
            driving mCherry GFP in lens.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Berger J, Currie PD
  TITLE     503unc, a small and muscle-specific zebrafish promoter.
  JOURNAL   Genesis. 2013 Jun;51(6):443-7. doi: 10.1002/dvg.22385. Epub 2013 Mar
            26.
  PUBMED    23444339
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/dvg.22385";
            journalName: "Genesis"; date: "2013-06"; volume: "51"; issue: "6"; 
            pages: "443-7"
COMMENT     Sequence Label: pDESTtol2pACrymCherry
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(85..91,92..109,110..115))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(140..263)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(271..287)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    303..437
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(327..346)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     381..400
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg
       61 acggatccta actcaaaatc cacacattat acgagccgga agcataaagt gtaaagcctg
      121 gggtgcctaa tgcggccgcc atagtgactg gatatgttgt gttttacagt attatgtagt
      181 ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg
      241 ttcaacttta ttatacatag ttgataattc actggccgtc gttttacggt accatcgatg
      301 atgatccaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
      361 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
      421 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
      481 aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg
      541 atcctctaga tcagatctgc gaagatacgg ccacgggtgc tcttgatcct gtggctgatt
      601 ttggactgtg ctgctcgcag ctgctgatga atcacatact tcctccattt tcttccactg
      661 attgactgtt ataatttccc taatttccag gtcaaggtgc tgtgcattgt ggtaatagat
      721 gtgacatgac gtcacttcca aaggaccaat gaacatgtct gaccaatttc atataatgtg
      781 aaaacgattt tcataggcag aataaataac atttaaatta aactgggcat cagcgcaatt
      841 caattgg
//
