LOCUS       sequence-109315-         740 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA for mammalian cells with mCherry marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Zalatan JG, Lee ME, Almeida R, Gilbert LA, Whitehead EH, La Russa M,
            Tsai JC, Weissman JS, Dueber JE, Qi LS, Lim WA
  TITLE     Engineering Complex Synthetic Transcriptional Programs with CRISPR 
            RNA Scaffolds.
  JOURNAL   Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052.
  PUBMED    25533786
REFERENCE   2  (bases 1 to 740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052."
COMMENT     Sequence Label: pJZC103
FEATURES             Location/Qualifiers
     source          1..740
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(144..347)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(173..193)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        348..651
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tcgatctcga actcgtggcc gttcacggag ccctccatgt gcaccttgaa gcgcatgaac
       61 tccttgatga tggccatgtt atcctcctcg cccttgctca ccccgggcat ggtgggctag
      121 cggatctgac ggttcactaa accagctctg cttatataga cctcccaccg tacacgccta
      181 ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt ttggaaagtc ccgttgattt
      241 tggtgccaaa acaaactccc attgacgtca atggggtgga gacttggaaa tccccgtgag
      301 tcaaaccgct atccacgccc attgatgtac tgccaaaacc gcatcaccat ggtaatagcg
      361 atgactaata cgtagatgta ctgccaagta ggaaagtccc ataaggtcat gtactgggca
      421 taatgccagg cgggccattt accgtcattg acgtcaatag ggggcgtact tggcatatga
      481 tacacttgat gtactgccaa gtgggcagtt taccgtaaat actccaccca ttgacgtcaa
      541 tggaaagtcc ctattggcgt tactatggga acatacgtca ttattgacgt caatgggcgg
      601 gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac gcggaactcc
      661 atatatgggc tatgaactaa tgaccccgta attgattact attaataact aatgcatggc
      721 ggtaatacgg ttatccacgc
//
