LOCUS       sequence-109317-         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Photoactivatable transcription system. Lentiviral expression of 
            NANOG sgRNA4. Also contains a CMV-puro-t2A-mCherry expression 
            cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Nihongaki Y, Yamamoto S, Kawano F, Suzuki H, Sato M
  TITLE     CRISPR-Cas9-based Photoactivatable Transcription System.
  JOURNAL   Chem Biol. 2015 Feb 19;22(2):169-74. doi: 
            10.1016/j.chembiol.2014.12.011. Epub 2015 Jan 22.
  PUBMED    25619936
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2014.12.011"; journalName: "Chem Biol"; date: 
            "2015-02-19- 19"; volume: "22"; issue: "2"; pages: "169-74"
COMMENT     Sequence Label: sgRNA4_NANOG
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        49..124
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        249..552
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        553..756
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     707..727
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(790..809)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 agtatccctt ggagaaccac cttgttggca tattcctgat ttaaaagtgt tttagagcta
       61 gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg
      121 gtgctttttt tctcgagtac taggatccat taggcggccg cgtggataac cgtattaccg
      181 ccatgcatta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
      241 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
      301 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
      361 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
      421 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
      481 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
      541 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
      601 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
      661 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
      721 aggcgtgtac ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc
      781 tagcccacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtccc
//
