LOCUS       sequence-109318-         601 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA (no RNA aptamer addition) with MCP-VP64 effector for mammalian
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Zalatan JG, Lee ME, Almeida R, Gilbert LA, Whitehead EH, La Russa M,
            Tsai JC, Weissman JS, Dueber JE, Qi LS, Lim WA
  TITLE     Engineering Complex Synthetic Transcriptional Programs with CRISPR 
            RNA Scaffolds.
  JOURNAL   Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052.
  PUBMED    25533786
REFERENCE   2  (bases 1 to 601)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052."
COMMENT     Sequence Label: pJZC32
FEATURES             Location/Qualifiers
     source          1..601
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             3..23
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             396..545
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
ORIGIN
        1 tgcccaaaaa gaaaagaaaa gtgggtagta tggcttctaa ctttactcag ttcgttctcg
       61 tcgacaatgg cggaactggc gacgtgactg tcgccccaag caacttcgct aacgggatcg
      121 ctgaatggat cagctctaac tcgcgttcac aggcttacaa agtaacctgt agcgttcgtc
      181 agagctctgc gcagaatcgc aaatacacca tcaaagtcga ggtgcctaaa ggcgcctggc
      241 gttcgtactt aaatatggaa ctaaccattc caattttcgc cacgaattcc gactgcgagc
      301 ttattgttaa ggcaatgcaa ggtctcctaa aagatggaaa cccgattccc tcagcaatcg
      361 cagcaaactc cggcatctac ggctccggac gagccgatgc tctcgacgat tttgacctcg
      421 atatgctggg atccgacgct ctcgatgact tcgaccttga catgctgggg agtgacgctc
      481 tggacgactt cgatctcgac atgctgggct ccgacgccct ggatgacttc gatctggaca
      541 tgctcatcaa ctgacccggg tcgagcatgc atctagggcg gccaattccg cccctctccc
      601 t
//
