LOCUS       sequence-109328-         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CrBlue  fused to mcherry on the C terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Karunarathne WK, Giri L, Kalyanaraman V, Gautam N
  TITLE     Optically triggering spatiotemporally confined GPCR activity in a 
            cell and programming neurite initiation and extension.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):E1565-74. doi: 
            10.1073/pnas.1220697110. Epub 2013 Mar 11.
  PUBMED    23479634
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1220697110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-04-23- 23"; volume: "110"; issue: "17"; pages: 
            "E1565-74"
COMMENT     Sequence Label: CrBlue-mcherry - pcDNA3.1
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     40..59
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        40..58
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggctactaga gaacccactg cttactggct tatcgaaatt aatacgactc actataggga
       61 gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc cactagtcca
      121 gtgtggtgga attcatgcgc aagatgtccg aggaggagtt ctacctgttc aagaacatct
      181 cgagcgtcgg cccttgggac gggccccagt accacatcgc tcctgtctgg gctttctacc
      241 tccaggctgc tttcatgggg accgtgttcc tcatcggttt ccctctgaat gccatggtgc
      301 tcgtggcctt ttaccgctta cgacataaat tggctttttt aaactacatc ctggtgaacg
      361 tgtccttcgg tggattcctg ctgtgtatct tctccgtgtt ccctgtgttc gtggctagct
      421 gcaacggtta cttcgtgttc ggacgtcacg tgtgtgctct ggagggtttc ctaggtaccg
      481 tggctggtct ggtcaccggg tggtctctgg ccttcctggc cttcgaacgc tacatcgtca
      541 tatgcagacc tttcgtggca actgcgattc acggatcaat gagaaatgcg ttaaccgtag
      601 tactggctac ctggacaatc ggtatcggtg tatcgatccc tcccttcttc ggttggtctc
      661 gcttcattcc ggaaggcctt cagtgcagct gtggccctga ctggtacacc gtgggtacaa
      721 agtaccgatc ggagagctac acctggttcc tgttcatctt ctgcttcatc gtgcctctga
      781 gcctgatctg cttctcctac accttggttc aaggagagat gaagaatatg cgaggtcgtg
      841 cagcacagtt gtttggttca gaatccgagg ctgcactaaa gaatatcaag gccg
//
