LOCUS       sequence-109329-         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CrBlue  fused to mcherry on the C terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Karunarathne WK, Giri L, Kalyanaraman V, Gautam N
  TITLE     Optically triggering spatiotemporally confined GPCR activity in a 
            cell and programming neurite initiation and extension.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):E1565-74. doi: 
            10.1073/pnas.1220697110. Epub 2013 Mar 11.
  PUBMED    23479634
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1220697110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-04-23- 23"; volume: "110"; issue: "17"; pages: 
            "E1565-74"
COMMENT     Sequence Label: CrBlue-mcherry - pcDNA3.1
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(268..287)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     537..555
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      697..706
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gctcgtccat gccgccggtg gagtggcggc cctcggcgcg ttcgtactgt tccacgatgg
       61 tgtagtcctc gttgtgggag gtgatgtcca acttgatgtt gacgttgtag gcgccgggca
      121 gctgcacggg cttcttggcc ttgtaggtgg tcttgacctc agcgtcgtag tggccgccgt
      181 ccttcagctt cagcctctgc ttgatctcgc ccttcagggc gccgtcctcg gggtacatcc
      241 gctcggagga ggcctcccag cccatggtct tcttctgcat tacggggccg tcggagggga
      301 agttggtgcc gcgcagcttc accttgtaga tgaactcgcc gtcctgcagg gaggagtcct
      361 gggtcacggt caccacgccg ccgtcctcga agttcatcac gcgctcccac ttgaagccct
      421 cggggaagga cagcttcaag tagtcgggga tgtcggcggg gtgcttcacg taggccttgg
      481 agccgtacat gaactgaggg gacaggatgt cccaggcgaa gggcaggggg ccacccttgg
      541 tcaccttcag cttggcggtc tgggtgccct cgtaggggcg gccctcgccc tcgccctcga
      601 tctcgaactc gtggccgttc acggagccct ccatgtgcac cttgaagcgc atgaactcct
      661 tgatgatggc catgttatcc tcctcgccct tgctcaccat ggcggccgca gcaggagcca
      721 cctgggaggt ctcgttaggt tcaacagccg tactggaagg agcaatggca ttaccaccga
      781 caattcttcc acaaacacca cgtaatacag ctctttggaa ctgcttgttc atgaagcagt
      841 agatgatagg gttgtaatcg atgcgactg
//
