LOCUS       sequence-109330-         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Photoactivatable transcription system. Expression of activator 
            probe, which includes the photolyase homology region of CRY2 
            (CRY2PHR) and the transcriptional activator domain..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Nihongaki Y, Yamamoto S, Kawano F, Suzuki H, Sato M
  TITLE     CRISPR-Cas9-based Photoactivatable Transcription System.
  JOURNAL   Chem Biol. 2015 Feb 19;22(2):169-74. doi: 
            10.1016/j.chembiol.2014.12.011. Epub 2015 Jan 22.
  PUBMED    25619936
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2014.12.011"; journalName: "Chem Biol"; date: 
            "2015-02-19- 19"; volume: "22"; issue: "2"; pages: "169-74"
COMMENT     Sequence Label: NLSx3-CRY2PHR-p65
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      81..90
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             96..116
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             132..152
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agttaagctt gccaccatgg ccagccccaa gaagaagcgc aaggtggaag
      121 ctagcgcttc cccgaagaaa aagcggaaag tcgaggcctc cgcatctcca aaaaaaaaaa
      181 gcaaggttga agcatctgga tccggtaccg gaggaagtgg cagctctggc ggcagtggag
      241 ggtctggtgg cagcggaatg aagatggaca aaaagactat agtttggttt agaagagacc
      301 taaggattga ggataatcct gcattagcag cagctgctca cgaaggatct gtttttcctg
      361 tcttcatttg gtgtcctgaa gaagaaggac agttttatcc tggaagagct tcaagatggt
      421 ggatgaaaca atcacttgct cacttatctc aatccttgaa ggctcttgga tctgacctca
      481 ctttaatcaa aacccacaac acgatttcag cgatcttgga ttgtatccgc gttaccggtg
      541 ctacaaaagt cgtctttaac cacctctatg atcctgtttc gttagttcgg gaccataccg
      601 taaaggagaa gctggtggaa cgtgggatct ctgtgcaaag ctacaatgga gatctattgt
      661 atgaaccgtg ggagatatac tgcgaaaagg gcaaaccttt tacgagtttc aattcttact
      721 ggaagaaatg cttagatatg tcgattgaat ccgttatgct tcctcctcct tggcggttga
      781 tgccaataac tgcagcggct gaagcgattt gg
//
